胱胺对野百合碱诱导的大鼠右心室肥厚的抑制作用

Inhibitory Effect of Cystamine on Right Ventricular Hypertrophy in Rats Induced by Monocrotaline

目的研究胱胺对野百合碱诱导的大鼠右心室肥厚的抑制作用。方法建立野百合碱诱导的肺动脉高压大鼠模型,用胱胺进行干预,观察大鼠肺动脉压力、右心室肥厚指数以及右心室与左心室+室间隔中Rho A 5-羟色胺化、蛋白激酶B(Akt)磷酸化的变化。结果野百合碱诱导大鼠肺动脉压力、右心室肥厚指数以及右心室中Akt磷酸化水平明显升高,胱胺抑制这些改变。而大鼠右心室与左心室+室间隔中Rho A 5-羟色胺化以及右心室中Akt磷酸化没有显著性变化。结论胱胺抑制野百合碱诱导的大鼠右心室肥厚,可能与Akt活性的抑制有关。

Objective To study the inhibitory effect of cystamine on right ventricular hypertrophy in rats induced by monocrotal-ine. Methods The rat model with pulmonary hypertension induced by monocrotaline was established. After treatment with cystamine for 3 weeks, pulmonary arterial pressure, right ventricular hypertrophy index, and RhoAserotonylation and protein kinase B （ Akt） phosphorylation in right ventricle, left ventricle and ventricular septum were measured. Results pulmonary artery pressure, right ventricular hypertrophy index and Akt phosphorylation in the right ventricle in rats induced by monocrotaline significantly increased, and cystamine inhibited these changes. However, RhoAserotonylation in the right ventricle, the left ventricle and ventricular septum, and Akt phosphorylation in the right ventricle did not change significantly. Conclusion Cystamine inhibits right ventricular hypertro-phy in rats induced by monocrotaline, which may be related to the inhibition of Akt activity.