大鼠胰岛素增强子结合蛋白ISL1的生物信息学分析

CHARACTERIZATION OF ISL1 PROTEIN FROM RATTUS NORVEGICUS USING BIOINFORMATICS TOOLS

以NCBI中收录的不同物种ISL1蛋白的相关序列信息为基础,进行生物信息学分析和功能预测。其中大鼠的ISL1基因长12307 bp,编码349个氨基酸。所编码蛋白质不含信号肽,可能是存在于内质网的亲水性蛋白。结构预测分析发现ISL1蛋白二级结构存在较多无规则卷曲和双锌指的空间结构,证实其发挥转录因子功能的结构基础。同源性比对分析显示,大鼠ISL1基因编码蛋白与人、奶牛、小鼠、金仓鼠、灰狼的ISL1蛋白高度同源,达到100%;与原鸡、斑马鱼、非洲爪蟾同源性也较高,分别达到99%、98%及97%,这说明了ISL1蛋白序列的稳定性和保守性。通过对大鼠ISL1蛋白的生物信息学分析和功能预测,为进一步探讨其分子调控机制提供重要的参考依据。

On the basis of the sequence information about ISL1 gene of different animals from NCBI, a series of bioinformatics analysis and function predictions was done about ISL1. Results revealed that the full-length of rat ISL1 gene was 12307 bp, encoding 349 amino acids. It was predicted that the ISL1 protein didn＇t have signal peptide and was a hydrophilic protein which was found in the endoplasmic reticulum. Structural prediction analysis revealed that the secondary structure of ISL1 protein had more random coil. The spatial structure resembled the double zinc finger that proved it could play a function as transcription factor. Homology comparison and phylogenetic analysis showed that the amino acid encoded by ISL1 gene in rat has highest homologies of 100% with those in human being, cow, mice, golden hamster and grey wolf. It was also highly homologous with those in Gullas gullas, Danio rerio, Xenopus laevis, with homologies of 99%, 98% and 97% respectively. The results show the stability and conservation of ISL1 protein sequence, and it could provide the theoretical foundation and reference for the subsequent experiment.