期刊文献+

应用光遗传学技术在体记录小鼠纹状体D1中型多棘神经元的活动模式 被引量:1

Recording of neuronal activity pattern of D1 medium spiny neurons in vivo with optogenetics
下载PDF
导出
摘要 目的:建立光遗传-在体多通道光电极记录系统,用其对直接通路中型多棘神经元(D1-MSN)的活动模式进行在体记录。方法:首先向D1-Cre转基因小鼠纹状体背外侧注射携带光敏阳离子通道channelrhodopsin-2(ChR2)基因的腺相关病毒,使ChR2在D1-MSN上通过Cre重组酶的同源重组而特异性表达。之后通过光刺激和电生理记录相结合的方式在体记录D1-MSN的活动模式。结果:D1-Cre小鼠纹状体在注射病毒后通过荧光显微镜观察,可发现明显的荧光信号,证明病毒正常表达。通过光遗传-在体多通道光电极记录系统,本研究成功地在纹状体内用光刺激诱发了MSN的电活动。通过对记录的电信号进行数据分析,证明了光刺激诱发的电信号确实来自于D1-MSN,成功地对D1-MSN活动模式进行了在体记录。结论:结果提示光遗传-在体多通道光电极记录系统是一种对纹状体D1-MSN电活动模式记录的可选新方法。 Objective: To record direct pathway medium spiny neurons( D1-MSN) in vivo. Methods: First,injected adeno-associated virus( AAV) which carried channelrhodopsin-2( ChR2) gene at the striatum of transgenic mice expressing Cre recombinase under control of regulatory elements for the dopamine D1 receptor. Then using optogenetics and in vivo multichannel electrophysiology to record MSN's activity patterns respectively and finally distinguish D1-MSN from other neurons. Results: With the AAV which carried DIO-ChR2-m Cherry,ChR2-m Cherry could be selectively expressed in D1-Cre mice's striatum. The electrophysiology results showed that action potentials of D1-MSN could be triggered by photostimulation in striatum could can be distinguished from background noise or other neuron. Conclusion: The optogenetics- in vivo multichannel optrode electrophysiology system can be used to record the activity patterns of D1-MSN in vivo.
出处 《神经解剖学杂志》 CAS CSCD 北大核心 2016年第5期573-578,共6页 Chinese Journal of Neuroanatomy
基金 国家自然科学基金(31171051) 北京市自然科学基金(5112008 5132007) 北京市教育委员会科技计划面上项目(KM201110025001)
关键词 在体多通道电生理 光遗传学 ChR2 基底神经节环路 中型多棘神经元 in vivo multichannel electrophysiology optogenetics ChR2 basal ganglia circuits medium spiny neuron
  • 相关文献

参考文献16

  • 1Utter A,Basso A.The basal ganglia:An overview of circuits and function[J].Neurosci Biobehav Rev,2008,32:333-342.
  • 2Ikemoto S,Yang C,Tan A.Basal ganglia circuit loops,dopamine and motivation:A review and enquiry[J].Behav Brain Res,2015,290:17-31.
  • 3Macpherson T,Morita M,Hikida T.Striatal direct and indirect pathways control decision-making behavior[J].Front Psychol,2014,5:1301-1307.
  • 4Cazorla M,Kang J,Kellendonk C.Balancing the basal ganglia circuitry:a possible new role for dopamine D2 receptors in health and disease[J].Mov Disord,2015,30:895-903.
  • 5Wei W,Rubin E,Wang J.Role of the indirect pathway of the basal ganglia in perceptual decision making[J].J Neurosci,2015,35:4052-4064.
  • 6Chuhma N,Tanaka KF,Hen R,et al.Functional connectome of the striatal medium spiny neuron[J].J Neurosci,2011,31:1183-1192.
  • 7Schroll H,Beste C,Hamker H.Combined lesions of direct and indirect basal ganglia pathways but not changes in dopamine levels explain learning deficits in patients with Huntington’s disease[J].Eur J Neurosci,2015,41:1227-1244.
  • 8Rodriguez M,Morales I,Gomez I,et al.Heterogeneous dopamine neurochemistry in the striatum:the fountain-drain matrix[J].J Pharmacol Exp Ther,2006,319:31-43.
  • 9Zhang F,Aravanis M,Adamantidis A,et al.Circuit-breakers:optical technologies for probing neural signals and systems[J].Nat Rev Neurosci,2007,8:577-581.
  • 10Grosenick L,Marshel H,Deisseroth K.Closed-loop and activityguided optogenetic control[J].Neuron,2015,86:106-139.

同被引文献6

引证文献1

二级引证文献4

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部