摘要
目的:建立光遗传-在体多通道光电极记录系统,用其对直接通路中型多棘神经元(D1-MSN)的活动模式进行在体记录。方法:首先向D1-Cre转基因小鼠纹状体背外侧注射携带光敏阳离子通道channelrhodopsin-2(ChR2)基因的腺相关病毒,使ChR2在D1-MSN上通过Cre重组酶的同源重组而特异性表达。之后通过光刺激和电生理记录相结合的方式在体记录D1-MSN的活动模式。结果:D1-Cre小鼠纹状体在注射病毒后通过荧光显微镜观察,可发现明显的荧光信号,证明病毒正常表达。通过光遗传-在体多通道光电极记录系统,本研究成功地在纹状体内用光刺激诱发了MSN的电活动。通过对记录的电信号进行数据分析,证明了光刺激诱发的电信号确实来自于D1-MSN,成功地对D1-MSN活动模式进行了在体记录。结论:结果提示光遗传-在体多通道光电极记录系统是一种对纹状体D1-MSN电活动模式记录的可选新方法。
Objective: To record direct pathway medium spiny neurons( D1-MSN) in vivo. Methods: First,injected adeno-associated virus( AAV) which carried channelrhodopsin-2( ChR2) gene at the striatum of transgenic mice expressing Cre recombinase under control of regulatory elements for the dopamine D1 receptor. Then using optogenetics and in vivo multichannel electrophysiology to record MSN's activity patterns respectively and finally distinguish D1-MSN from other neurons. Results: With the AAV which carried DIO-ChR2-m Cherry,ChR2-m Cherry could be selectively expressed in D1-Cre mice's striatum. The electrophysiology results showed that action potentials of D1-MSN could be triggered by photostimulation in striatum could can be distinguished from background noise or other neuron. Conclusion: The optogenetics- in vivo multichannel optrode electrophysiology system can be used to record the activity patterns of D1-MSN in vivo.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2016年第5期573-578,共6页
Chinese Journal of Neuroanatomy
基金
国家自然科学基金(31171051)
北京市自然科学基金(5112008
5132007)
北京市教育委员会科技计划面上项目(KM201110025001)