急性应激对大鼠纹状体nNOS表达及分布的影响

Effect of acute stress on nNOS expression and distribution in the striatum of rats

目的:研究应激对大鼠纹状体内神经型一氧化氮合酶(neuronal nitric oxide synthase,nNOS)表达和分布的影响。方法:建立大鼠运输应激模型(120 r/min,持续2 h),将24只大鼠随机分为对照组和应激组。应用Western Blot分别测定对照组和应激组大鼠脑内nNOS的表达量并结合免疫细胞化学染色探察应激前后纹状体nNOS神经元的分布及其形态学特征的变化。结果:Western Blot结果显示应激后nNOS在蛋白水平上显著提高(P<0.05);免疫组化染色显示nNOS阳性神经元在纹状体呈散在分布,可见棕色免疫阳性物质主要位于细胞质中,细胞核着色浅,细胞轮廓清晰,突起明显;与对照组相比,应激组阳性细胞数显著增多(P<0.05),且着色较深。结论:应激使大鼠纹状体内nNOS表达显著增多,nNOS的升高可能是引起运输焦虑的深层原因之一。

Objective： To explore the effect of transport stress on expression and distribution of neuronal nitric oxide synthase（ nNOS） in the rat striatum. Methods： Transport stress model（ 120 r / min,continuous stress 2 h）,24 rats were randomly divided into control group and stress group. The expression of nNOS was examined in the rat striatum by Western blot,and the distribution and morphological features of nNOS neurons were further investegatied by using immunocytochemistry. Results： The results showed that nNOS protein level significantly increased after stress. nNOS-positive neurons were scattered throughthe striatum. Immune positive material showed claybank,which mainly located in the cytoplasm. The nucleus was less stained,contour wasclear,protuberance can be seen. Compared with control group,the number of positive cells and the expression of positive material significantly increased in stress group. Conclusion： Transport stress significantly increased nNOS expression（ P〈0. 05） in rats striatum,the increased nNOS may be one of the deep reasons why transport stress cause animal anxiety.