APS通过PI3K/AKT信号通路促进神经干细胞增殖

Astragalus polysaccharide( APS) promotes proliferation of neural stem cells(NSCs) through PI3K/AKT signaling pathway

目的观察APS是否通过PI3K/AKT信号通路促进神经干细胞增殖。方法将神经干细胞,随机分为对照组、APS(5%)组、APS(5%)+LY294002组和LY294002组,用间接免疫荧光法检测Nestin和Brdu;CCK-8检测细胞增殖;Elisa检测VEGF、FLK-1含量;Western blot法检测FLK-1、PI3K、P-Akt蛋白表达。结果 APS可以提高LY294002干预后神经干细胞增殖(P<0.05),提高VEGF、FLK-1含量(P<0.05),提高FLK-1、PI3K、P-Akt蛋白表达(P<0.05)。结论 APS可能通过促进Akt磷酸化,活化PI3K/AKT信号通路,调节PI3K/AKT信号通路下游靶基因,促进神经干细胞增殖。

Objective To observe the effect of astragalus polysaccharide on the proliferation of neural stem cells through FLK-1 / PI3 K / AKT signaling pathway. Methods Neural stem cells were randomly divided into control group,APS（ 5%） group,APS（ 5%） ＋ LY294002 group,LY294002 group. We detected the expression of Nestin and Brdu; Tested the cell proliferation by CCK-8; the content of VEGF,FLK-1 by ELISA,and the protein expression of FLK-1、PI3K、P-Akt by Western blot. Results The proliferation of neural stem cells in APS group and LY294002 group was significantly different（ P 〈0. 05）,APS can improve the proliferation of neural stem cells after LY294002 intervention,and increase the content of VEGF,FLK-1 and FLK-1,P-Akt and PI3 K protein expression（ PC）. Conclusions APS group can promote the proliferation of neural stem cells,maybe related with improvement of content of VEGF,FLK-1,promotion of the expression of FLK-1 protein expression,Akt phosphorylation,activate PI3 K / AKT signaling pathway,regulate the downstream target genes of PI3 K / AKT signaling pathway.