胶原三螺旋重复蛋白1在胃癌组织中的表达及其临床意义

Expression and clinical significance of collagen three helix repeat protein 1 in human gastric carcinoma

目的：探讨胶原三螺旋重复蛋白1（ CTHRC1）在胃癌组织中的表达及其与胃癌临床病理特征的关系。方法采用免疫组织化学方法（ S.P法）检测胃癌组织和正常胃黏膜组织中CTHRC1的表达水平，分析其表达与患者性别、年龄、肿瘤直径、分化程度、浸润深度、淋巴结转移、TNM分期等临床病理特征的关系。结果（1）76例患者胃癌组织中CTHRC1阳性表达41例（53.95％，41／76），30例正常胃黏膜组织中仅有1例CTHRC1阳性表达（3.33％，1／30），两组比较差异有统计学意义（χ2＝23.0332，P＝0.000）。（2）在早期胃癌中，CTHRC1多表达于细胞核，随着胃癌的进展，CTHRC1在胞浆中的表达逐渐增加。（3）CTHRC1蛋白在胃癌中的表达与肿瘤浸润深度（P＝0.000）、淋巴结转移（P＝0.009）、TNM分期（ P＝0.007）密切相关，而与年龄、性别、肿瘤直径及肿瘤分化程度无关（ P 均＞0.05）。结论CTHRC1可能在胃癌的发生发展及浸润转移过程中发挥重要作用，有望成为胃癌新的治疗靶点。

Objective To investigate the expression level of collagen triple helix repeat containing 1 （ CTHRC1） in human gastric carcinoma and the relationship with the clinicopathological characteristics of gastric cancer.Methods The expression of CTHRC1 in human gastric carcinoma and normal gastric mucosa were detected by immunohistochemistry （ S.P method ） , and the correlation with various clinical characteristics, including gender,age,tumor diameter,degree of differentiation,depth of invasion,lymph node metastasis,TNM stage,was analyzed.Results （ 1 ） CTHRC1 expressed positive for 41 cases （ positive rate=53.95%） in 76 gastric carcinoma specimens, but only 1 case （ positive rate=3.33%） expressed positive in 30 normal gastric mucosa,the difference was statistically significant （χ2 =23.0332, P=0.000 ）. （ 2 ） In early stage of gastric carcinoma,CTHRC1 was predominantly positive in the nucleus,but with the progression of the tumor,CTHRC1 expressed predominantly in cytoplasm.（ 3） The expression of CTHRC1 was correlated with the depth of invasion （P=0.000）,lymph node metastasis（P=0.009） and TNM.stage（P=0.007）,but not with age,gender,size of the tumor and differentiated degree （ P〉0.05 ）.Conclusion CTHRC1 might play important roles in the occurrence,invasion and metastasis in human gastric carcinoma,and may be new therapy targets.