摘要
目的探讨miRNA-29b在可手术的肺鳞癌组织中的表达及其与转移及预后的关系。方法回顾性分析2006年6月至2013年12月间武警总医院与中国医科大学附属第一医院62例肺鳞癌手术患者的临床资料,采用RT-PCR方法检测上述病例肺鳞癌组织标本中miRNA-29b的表达水平,根据miRNA-29b相对表达量进行实验分组。应用x^2检验分析miRNA-29b表达与肺鳞癌患者转移的关系,应用Kaplan-Meier方法分析miRNA-29b表达与生存的关系,应用Cox风险比例模型分析miRNA-29b表达与预后的关系,应用Western blot分析肺鳞癌组织标本中胰岛素样生长因子-1(IGF1),分析miRNA-29b与IGF1表达的相关性。结果肺鳞癌组织中miRNA-29b的表达量显著低于正常肺组织,与转移相关(P<0.05),低表达组生存期较高表达组明显缩短(P<0.01),miRNA-29b表达可作为肺鳞癌患者预后的独立影响因素。IGF1在肺鳞癌组织标本中的表达显著高于正常肺组织(P<0.01)。结论 miRNA-29b低表达与非小细胞肺癌转移及生存期相关,miRNA-29b表达与IGF1的表达呈显著负相关。
Objective To investigate the expression of miRNA-29b in lung squamous cell carcinoma and its correlation with prognosis. Methods The clinical data of 62 cases of lung squamous cell carcinoma patients treated by surgery in Liaoning Provincial Corps Hospital of Chinese People＇ s Armed Police Forces and the First Affiliated Hospital of China Medical University from June 2006 to December 2013 were retrospectively analyzed. The expression of miRNA-29b in lung squamous cell carcinoma was detected by quantitative RT-PCR, the correlation of miRNA-29b expression with the clinical pathological parameters of lung squamous cell carcinoma patients was analyzed by X 2 test. Kaplan-Meier method was used to analyze the relationship between miRNA-29b expression and survival of lung squamous cell carcinoma, independent factor of prognosis was analyzed by Cox proportional hazards model. The expression of insulin-like growth factor-1（IGF1） in lung squamous cell carcinoma and normal tissues was detected by Western blot, and the correlation analysis between the expression of miRNA-29b and IGFlwas performed. Results The expression level of miRNA-29b was remarkably lower in lung squamous cell carcinoma tissues than in normal tissues, and was associated with metastasis （P〈0.05）. The survival time was shorter in low expression of miRNA-29b group than in high expression group（P〈0.01）, miRNA-29b could be an independent factor for prognosis of patients with non-small cell lung cancer. The expression level of IGF1 was significantly higher in cancer tissues than in normal tissues（P〈0.01）, with negative correlation between IGF1 and miRNA-29b. Conclusion The expression of miRNA-29b was related to the metastasis and poor prognosis of lung squamous cell carcinoma, with negative correlation between IGF 1 and miRNA-29b.
出处
《解剖科学进展》
2016年第5期493-496,共4页
Progress of Anatomical Sciences
基金
辽宁省自然科学基金(2015020561)
武警部队后勤科研项目(WJHQ2012-19)
中国医科大学附属第一医院资助项目(fsfh1514)
