摘要
以当年生长良好的牛耳朵叶片为外植体,通过对外植体的消毒、不定芽诱导、增殖及生根培养,筛选高效稳定的无菌芽诱导优化体系。结果表明:外植体最佳消毒方法为用70%酒精浸泡30s,3%Na Cl O消毒2.0 min;不定芽诱导最佳培养基为:WPM+6-BA3.0mg/L+2-ip 1.0mg/L+NAA0.1mg/L,诱导率为83.23%;不定芽增殖最佳培养基为MS+6-BA1.0mg/L+NAA0.1mg/L+GA30.5mg/L,增殖系数为16.0,且不定芽长势良好;生根培养基以1/2MS+IBA0.1mg/L+NAA0.05mg/L为较好,生根率达92.35%。以腐殖土:珍珠岩=3:1为移栽基质的成活率最高达100%。
The shoots were induced in vitro from Chirita eburnean Hanc leaflets through disinfection,adventitious shoots induction,proliferation and rooting culture. The results showed that the best method for the explant disinfection was '30s soaked in 70% alcohol',3% Na Cl O disinfection 2min; The best medium for adventitious bud induction: WPM + 6-BA3. 0mg/L 2-ip 1. 0mg/L NAA0. 1mg/L,the induction rate was 83. 23%; The best medium for adventitious bud proliferation of MS + 6-BA1. 0mg/L NAA0. 1mg/L GA30. 5mg/L,the proliferation coefficient was 16,and the adventitious buds grew well; 1/2MS + IBA0. 1mg/L NAA0. 05 mg/L was the best rooting medium,and the rooting rate was 92. 35%. In the humus soil: Perlite = 3: 1 for transplanting survival rate was as high as 100%.
出处
《贵州林业科技》
2016年第3期30-34,共5页
Guizhou Forestry Science and Technology
基金
贵州省科技厅推广项目"贵州野生草本花卉引种栽培示范"(编号:黔科合成字(2012)5033号资助)
关键词
牛耳朵
芽诱导
培养基
Chirita eburnean Hanc
Shoots induction
Medium
