血瘀质非创伤性股骨头坏死基因多态性研究

Study on Polymorphism of Nontraumatic Osteonecrosis of Femoral Head in Blood Stasis

目的研究非创伤性股骨头坏死(nontraumatic osteonecrosis of femoral head,NONFH)高发体质类型血瘀质与Apo A1位点多态性的相关性。方法选择93例NONFH患者作为病例组,随机选取无血缘关系健康志愿者83例作为对照组。对病例组进行中医体质问卷调查,将NONFH患者分为血瘀质32例、非血瘀质61例。受试者取血样2 m L,提取DNA进行PCR扩增,对PCR产物进行DNA测序,检测PAF-AH G994T、NOS1基因rs9658282位点多态性,进行统计学分析。结果 Apo A1基因-75G/A位点AA基因型(OR:2.578;95%CI:1.174-5.663;P=0.018)与A等位基因(OR:1.726;95%CI:1.121-2.658;P=0.013)可能是NONFH发病的危险因素之一。结论 Apo A1基因-75G/A可能与NONFH发病相关。Apo A1基因-75G/A位点基因多态性与血瘀质NONFH发病无相关性,Apo A1基因+83C/T位点基因多态性与血瘀质NONFH发病无相关性。

Objective To study the correlation between nontraumatic osteonecrosis of femoral head（NONFH） and Apo A1 polymorphism of blood stasis type. Methods Totally 93 cases of NONFH were selected as the case group, and 83 healthy volunteers were randomly selected as the control group. With TCM constitution questionnaire survey, the case group was screened out 32 cases of blood stasis NONFH type and 61 cases of non blood stasis NONFH type. In the case group and control group, the subjects took blood samples 2 m L, extracted DNA for PCR amplification, PCR products for DNA sequencing. G994 T PAF-AH and rs9658282 gene NOS1 site polymorphism were detected for tatistical analysis. Results-75G/A gene AA Apo A1 genotype（OR： 2.578; 95%CI： 1.174-5.663; P=0.018） and A allele（OR： 1.726; 95%CI： 1.121-2.658; P=0.013） may be one of the risk factors of NONFH. Conclusion-75G/A gene Apo A1 may be related to the pathogenesis of NONFH. There was no correlation between the Apo A1 gene polymorphism of-75G/A gene and the pathogenesis of blood stasis NONFH. There was no correlation between the Apo A1 gene polymorphism of ＋83C/T gene and the pathogenesis of blood stasis NONFH.