当归挥发油对高血压模型大鼠血压及血管炎症反应的影响

Effects of Volatile Oil of Angelicae Sinensis Radix on Blood Pressure and Vascular Inflammatory Response in Hypertensive Rat Models

目的观察当归挥发油对高血压模型大鼠血压和相关炎症因子的影响,探讨其降压的作用机制。方法采用N-硝基-L-精氨酸灌胃建立高血压大鼠模型。实验大鼠随机分为正常组、模型组、阳性药组和当归挥发油高、低剂量组,每组5只。各给药组给予相应药物灌胃,连续8周。采用无创血压检测系统检测给药前后大鼠尾动脉收缩压;ELISA检测大鼠血清内皮素-1(ET-1)、血管细胞黏附分子-1(VCAM-1)、超敏C反应蛋白(CRP)水平;实时荧光定量PCR检测心脏组织CRP m RNA表达。结果与正常组比较,模型组收缩压、ET-1、VCAM-1、CRP水平及其m RNA表达明显升高(P<0.05,P<0.01);与模型组比较,各给药组ET-1、VCAM-1、CRP水平及其m RNA表达明显降低(P<0.05,P<0.01)。结论当归挥发油可能通过抑制血管炎症反应起到降压作用。

Objective To observe the effects of volatile oil of Angelicae Sinensis Radix on blood pressure and related inflammatory cytokines of hypertensive rat models; To investigate the action mechanism of regulating the blood pressure. Methods Wistar male rats were fed with L-NNA to establish hypertensive rat models. Hypertensive rats were randomly divided into normal control group, model group, positive medicine group, volatile oil of Angelicae Sinensis Radix high- and low-dose groups, with 5 rats in each group. Each medication group was given relative medicine for gavage for 8 weeks. Non-invasive blood pressure measurement system was used to observe the systolic pressure of tail artery of rats before and after medication; the levels of ET-1, CRP, and VCAM-1 in serum were detected by the method of ELISA; RT-PCR was used to measure CRP m RNA of heart. Results The levels of systolic blood pressure, ET-1, VCAM-1, CRP and CRP m RNA in model group were significantly higher than those of normal group（P〈0.05, P〈0.01）; Compared with model group, the levels of ET-1, VCAM-1, CRP and CRP m RNA in model group significantly decreased（P〈0.05, P〈0.01）. Conclusion Volatile oil of Angelicae Sinensis Radix can inhibit vascular inflammatory response and then realize the effects of regulating blood pressure.