黄连对脾虚大鼠胃肠运动功能及胃动素、胃泌素的影响

Effects of Coptidis Rhizoma on Gastrointestinal Motility Functions, MTL and GAS in Spleen-deficiency Rats

目的观察黄连对脾虚大鼠胃肠运动功能及胃动素(MTL)、胃泌素(GAS)的影响,探讨其"健胃""败胃"的量效机制。方法采用劳形与劳神制作劳倦过度脾虚模型。60只SD大鼠随机分为空白对照组、模型组、阳性药组和黄连大、中、小剂量组,每组10只。各给药组给予相应药液灌胃,空白对照组和模型组给予生理盐水灌胃,每日1次,连续14 d。观察各组脾虚大鼠一般情况、胃肠运动功能及MTL、GAS含量。结果与空白对照组比较,模型组大鼠胃内残留率明显升高,小肠推进率、MTL和GAS含量明显降低(P<0.05,P<0.01);与模型组比较,黄连小剂量组胃内残留率明显降低,小肠推进率、MTL和GAS含量明显升高(P<0.05,P<0.01);黄连大剂量组小肠推进率、MTL含量明显降低(P<0.05)。结论黄连大剂量"败胃"、小剂量"健胃",其量效机制可能与直接调节胃肠运动,或通过调节胃肠激素的分泌调节胃肠运动有关。

Objective To observe the effects of Coptidis Rhizoma on gastrointestinal motility functions, MTL and GAS in spleen-deficiency rats; To explore the dose-effect mechanism of ＂invigorating stomach＂ and ＂spoiling appetite＂. Methods Fatigue and troublesome were used to establish exhausted spleen-deficiency models. 60 SD rats were randomly divided into blank control group, model group, positive medicine group, Coptidis Rhizoma high-, medium- and low-dose groups, with 10 rats in each group. Each medication group received relevant medicine for gavage, while the blank control group and model group received normal saline for gavage, once a day for 14 d. The general gastrointestinal motility functions, MTL and GAS of rats in each group were observed. Results Compared with blank control group, the gastric residual rate of model group was significantly higher; small intestinal propulsion rate, MTL, and GAS were significantly lower（P〈0.05, P〈0.01）. Compared with model group, the gastric residual rate in Coptidis Rhizoma low-dose group was significantly lower; small intestinal propulsion rate, MTL and GAS increased significantly（P〈0.05, P〈0.01）; small intestinal propulsion rate and MTL in Coptidis Rhizoma high-dose group were significantly lower（P〈0.05）. Conclusion High-dose Coptidis Rhizoma leads to ＂spoiling appetite＂, and low-dose Coptidis Rhizoma leads to ＂invigorating stomach＂, which may be related to adjusting gastrointestinal motility directly or adjusting the secretion of gastrointestinal hormones.