小鼠肾脏中鼠疫菌的分离结果与分析

Results and analysis of isolated Yersinia pestis from the mice' kidney

培养28℃弱毒株鼠疫菌EV_(76),分别稀释为50亿/mI、100亿/mL、200亿/mL三个剂量组对成体小白鼠经皮下感染,对感染后死亡的小白鼠进行解剖,分别取淋巴结、肝脏、脾脏、肾脏、肺脏、心脏及骨髓进行鼠疫细菌学培养、鼠疫噬菌体裂解实验。结果显示各脏器鼠疫菌的分离率淋巴、肾脏、脾脏较高,分别为71.43%,73.33%,80.00%。从本实验结论表明,鼠疫菌经皮下感染小鼠后肾脏检出菌率较高,介于脾脏和淋巴之间,且噬菌体裂解实验为阳性,建议在野外工作中考虑分离肾脏中的鼠疫菌分离。

The results of the viscera tissue ’s bacteria culture under cultivated 28 ℃ were observed and phage ly-sis test was implemented. The difference of the viscera tissue under different concentration was analyzed. The attenua-ted strain Yersinia pestisKV76 was diluted to 200 亿 /mL, 100亿/mL, 50亿/mL respectively , infected adult mice via subcutaneous infection, after infected, the dead mice were dissected and sampled lymph node, liver, spleen, kid-ney, lung, heart and marrow respectively , and conducted plague bacterial culture and bacteriophage lysis test. The experiment results showed that isolated rate of plague bacteria in the lymph, kidney , spleen were higher than others organs, reach 71. 43% , 73. 33% , 80. 00% respectively . It conclude that the traditional bacterial culture only con-siders the heart, liver, spleen, lung, s bacteria isolation. In this experiment it is suggested that considered the isola-tion of Y. pestis in kidney in field work.