壳聚糖修饰的L-天门冬酰胺酶脂质体的稳定性

Preliminary Study on the Stability of Chitosan-Modified Liposomes Containing L-Asparaginase

考察壳聚糖修饰的L-天门冬酰胺酶脂质体在体外的稳定性。分别从最适pH、最适温度、储存稳定性、酸碱稳定性、热稳定性,以及抗胰蛋白酶水解的能力等方面,对比游离L-天门冬酰胺酶及壳聚糖修饰的L-天门冬酰胺酶脂质体。结果表明:游离L-天门冬酰胺酶和壳聚糖修饰的L-天门冬酰胺酶脂质体的最适pH分别为7.5和7.0,最适温度分别为60℃和50℃,壳聚糖修饰的L-天门冬酰胺酶脂质体的储存稳定性、酸碱稳定性、热稳定性,以及抗胰蛋白酶水解的能力均优于游离酶。因此,壳聚糖修饰的L-天门冬酰胺酶脂质体能明显提高游离酶的稳定性。

To investigate the in vitro stability of chitosan-modified liposomes containing L-asparaginase （LACL） ,the optimal pH,temperature,storage stability,pH stability,thermal stability and proteolytic stability were investigated in this study. The optimal pH values of L-ASP and LACL were at 7.5 and 7.0,respectively. The optimal temperatures of L-ASP and LACL were at 60 ℃ and 50 ℃ respectively. The stabilities of LACL were higher than that of L-ASP. The results shown that the stability of L-ASP was significantly enhanced by the LACL.