维生素A水平与儿童肺炎支原体感染的关系

The relationship between the vitamin A level and Mycoplasma pneumonia in children

目的 探讨维生素A （VA） 水平与肺炎支原体 （MP） 感染的相关性.方法 随机选取2015年3月-2015年12月因呼吸道急性感染性疾病住院的0-12岁儿童为研究对象.采用高效液相色谱法 （HPLC） 检测血清VA水平, 聚合酶链反应 （PCR） 检测鼻咽拭子MP-DNA, 酶联免疫吸附法 （ELISA） 检测血清MP-IgM.分析和比较亚临床VA缺乏 （SVAD）、可疑亚临床维生素A缺乏 （SSVAD） 和VA正常患儿间MP感染情况.结果 600例患儿中SVAD 83例 （13.83%）, SSVAD 193例 （32.17%）, 〈 1岁、 1-3岁、 6岁以及-〉 6岁组间SVAD及SSVAD发生率的差异有统计学意义 （P均 〈 0.001）, 其中 〈 1岁婴儿SVAD及SSVAD发生率最高, 分别为26.36%和49.10%. 600例患儿中MP阳性病例201例 （33.5%）, 其中SVAD 57例 （28.35%）, SSVAD 70例 （34.83%）; MP阳性患儿的SVAD发生率高于阴性患儿, 差异有统计学意义 （P〈 0.001）.201例MP阳性患儿不同年龄组间SVAD、 SSVAD以及VA正常儿童分布的差异有统计学意义 （P = 0.003）, 〈 1岁婴儿SVAD发生率 （48.39%） 较高. 结论 SSVAD、 SVAD以 〈 1岁婴儿最多, SVAD与儿童MP感染可能相关.

Objective To explore the correlation between vitamin A （VA） level and Mycoplasma pneumonia （MP）. Methods Children aged 0-12 years hospitalized with acute infectious respiratory diseases during March 2015 to December 2015 were randomly selected. The level of serum VA was detected by high performance liquid chromatography （HPLC）. MP-DNA on nasopharyngeal swab was detected by polymerase chain reaction （PCR）. The serum MP-IgM was detected by enzyme linked immunosorbent assay （ELISA）. The MP infection rate among subclinical VA deficiency （SVAD） group, suspicious subclinical VA defciency （SSVAD） group and VA normal group were analyzed and compared. Results Among 600 children, there were 83 cases of SVAD （13.83%） and 193 cases of SSVAD （32.17%）. There were statistical differences of the incidences between SVAD and SSVAD in children younger than 1-year-old, 1-3 years old, 3-6 years old and ≥6 years old （P all 〈 0.001）, among which SVAD and SSVAD groups had the highest incidence rates in infants younger than 1 year old （26.36% and 49.10% respectively）. Among 600 children, MP was positive in 201 children （33.5%）, in whom 57 children （28.35%） were SVAD and 70 children （34.83%） were SSVAD. The incidence rate of SVAD in children with MP positive was higher than that in children with MP negative （P 〈 0.001）. In 201 children with MP positive, there were signifcant differences in the distribution of SVAD, SSVAD and VA among different age groups （P =0.003）, and the incidence rate of SVAD in infants younger than 1 year old was higher （48.39%）. Conclusions SSVAD and SVAD are common in infants younger than 1 year old; SVAD may be associated with MP infection in children.