碱性磷酸酶同工酶在原发性肺癌骨转移诊断中的应用

The application of alkaline phosphatase isoenzyme in the diagnosis of bone metastasis of lung carcinoma

目的:探讨骨碱性磷酸酶同工酶(bone alkaline phosphatase,BALP)与原发性肺癌骨转移的相关性。方法:采用神经氨酸苷酶处理血清,琼脂糖电泳法分析测定50名健康体检者和131例原发性肺癌患者血清中的血清碱性磷酸酶(ALP)同工酶。结果:原发性肺癌组的总ALP和同工酶均高于正常对照组,原发性肺癌骨转移组的总ALP和BALP活性均显著高于对照组和无骨转移组,原发性肺癌无骨转移组的总ALP和BALP活性和对照组无显著性差异。结论:骨型ALP与原发性肺癌骨转移有一定的相关性,经神经氨酸苷酶处理血清后,采用琼脂糖电泳法分离ALP同工酶有助于临床辅助诊断原发性肺癌有无骨转移。

Objective： To discuss the relationship of BALP isoenzyme and metastasis of lung carcinoma. Methods：ALP isoenzyme in 50 normal human and 131 lung tumors were analyzed by agarose electrophoresis after using preincubated sera with neuraminidase. Results： Total ALP and isoenzyme in tumor group were higher than in normal group;BALP and total ALP in group of bone metastasis of lung carcinoma were higher than in the normal group,there were no difference in BALP and total ALP between no bone metastasis of lung carcinoma and normal group. BALP and total ALP in bone metastasis of lung carcinoma were higher than no bone metastasis. Conclusion： There was relationship between bone ALP and bone metastasis of lung carcinoma,also between bone ALP and bone metastasis of carcinoma.After using preincubated sera with neuraminidase,agarose gel electrophoresis separation of ALP isozymes contribute to the clinical diagnosis of lung cancer with and without bone metastasis.