高产过氧化氢酶菌株的鉴定与产酶条件

Identification and Optimized Fermentation of High Catalase-producing Serratia marcescens,FZSF02

为解决过氧化氢废水对环境的污染,从稻田土中筛选到1株高产过氧化氢酶菌株。经形态学观察和分子生物学鉴定,确认该菌属于黏质沙雷氏菌,命名为Serratia marcescens FZSF02。通过培养基碳源、氮源、无机盐类型及产过氧化-氢酶条件优化。在培养条件为:1%大豆蛋白胨、0.5%麦芽糖、0.2%NaCl、31℃、180r·min^(-1)、发酵时间48h,菌株产过氧化氢酶总酶活最高达6 380U·mL^(-1),比优化前提高了7.4倍。粗酶液最适反应温度为60℃,最适反应pH为8.0。该菌在过氧化氢废水无害化处理和其他工农业过程中有很好的应用潜力。

To search for a high catalase-producing bacteria strain,field samples and lab collections were screened.FZSF02 was initially selected and subjected to morphological examinations and 16 SrRNA sequencing.It was identified to be a strain of Serratia marcescens.Subsequently,various carbon and nitrogen sources,mineral salts,and fermentation conditions to maximize the catalase production of the bacteria were experimented and optimized.It was found that a medium consisting of 1%soy peptone,0.5%lactose,and 0.2% NaCl applied for the fermentation at 31℃ with a constant shaking at 180 rpm for 48 hcould yield the enzyme at the highest level of 6 380U·mL^（-1）among all tested conditions.The yield was 7.4times of what was without the optimization.The peak activity of the obtained crude catalase was determined to reach at 60℃ and pH 8.0.The enzyme could potentially be applied for treating hydrogen peroxide pollutionin waste water effluent.