鱼腥蓝细菌PCC 7120异形胞的分离

Isolating heterocysts fromAnabaena sp.strain PCC 7120

鱼腥蓝细菌PCC 7120是一种光合自养型丝状蓝细菌。缺氮条件下,菌丝上5%-10%的营养细胞分化为异形胞,发挥固氮作用。异形胞是研究生物固氮和细胞分化的理想材料。为研究异形胞生理功能及其发育过程中的基因表达变化,需要从菌丝中分离高质量的异形胞。用2mg/mL溶菌酶与0.1%Triton X-100同时处理菌丝30min,得到了完整性好、纯度高的异形胞。

The filamentous cyanobacterium Anabaena sp. strain PCC 7120 is able to undergo cell differentiation. When facing nitrogen deficiency,5 % -10 % vegetative cells will develop into heterocysts for fixing nitrogen.Thus this strain is an excellent material for studying nitrogen fixation and prokaryotic cell differentiation.Isolation of high quality heterocysts is a prerequisite to study their physiological chan-ges and regulation of related gene.However,currently available methods for isolating heterocyst are usually time-consuming, low yield and low purity , unable to fullfill the requirements of many researches. An efficient method for isolating heterocyst was developed, in which vegetative cells were effectively broken in the presence of 2 mg/mL lysozyme and 0.1 % Triton X-1 00 , allowing heterocysts to be enriched with high purity and good integrity.This method will greatly facilitate heterocyst-related studies.