益生屎肠球菌HDRsEf1抗氧化性能评估

Antioxidant performance evaluation of Enterococcus faecium HDRsEf1

采用过氧化氢耐受性实验、清除自由基(DPPH自由基、羟自由基、超氧自由基)实验、还原性实验,评价屎肠球菌HDRsEf1完整细胞、无细胞提取物、发酵液在体外的抗氧化性能;并通过构建D-半乳糖小鼠衰老模型评价屎肠球菌HDRsEf1在体内的抗氧化性能。结果显示:屎肠球菌HDRsEf1对过氧化氢溶液具有较好的耐受性;发酵液不仅对DPPH自由基具有很好的清除能力,而且也具有很好的还原能力;发酵液、无细胞提取物和菌悬液具有很强的羟自由基清除能力,但对超氧自由基清除能力较弱;屎肠球菌HDRsEf1菌株、发酵液均能显著提高小鼠血清、脑内超氧岐化酶(SOD)活力,降低脂肪质过氧化产物(MDA)含量。因此,屎肠球菌HDRsEf1在体外和体内都具有很好的抗氧化性。

For the impact of oxidative stress on animal husbandry industry, this study aimed to investigate the antioxidant property of Enterococcus faecium HDRsEf1 , which would provide a new method for development of new biological antioxidants.To assess antioxidant activity of the strain in vitro 9 the resistance to hydrogen peroxide,scavenging activity of the free radical （DPPH free radical, hydroxyl radical and superoxide radical） ,and reducing activity were analyzed. And to evaluate the antioxidant property of the E. faecium HDRsEf1 in vivo ,the D-galactose induced aging model were built.The results showed that the strain could survive under 2. 0 mmol/L of hydrogen peroxide. The fermentation broth of HDRsEf1 not only had good scavenging activity of the DPPH free radical but also had a strong reducing activity.The intact cell,cell-free extract,fermentation broth of E . faecium HDRsEfl also displayed good scavenging activity of hydroxyl radical, but weak superoxide radical.The experimental investigation demonstrated that the cell and fermentation broth of E . faecium HDRsEfl displayed effective function of antioxidation and antiaging by increasing the activities of superoxide dismutase （SOD） and decreasing the malondialdehyde （MDA） level prominently in the D-galaetose treated mice.Therefore, E. faecium HDRsEfl had good antioxidant activity in vitro and in vivo.