热疗联合5氟尿嘧啶对裸鼠移植性MKN45胃癌细胞凋亡的研究被引量：1

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摘要目的探讨热疗联合5-氟尿嘧啶(5-FU)化疗对小鼠MKN45肿瘤模型细胞凋亡的影响。方法建立裸鼠模型,分为空白对照组、5-FU、热疗组和热疗联合5-FU。流式细胞仪测定凋亡率,免疫组化分析技术(ICH)测定瘤组织表皮细胞生长因子受体(EGFR)和凋亡抑制因子Bcl-2蛋白表达变化。结果:热疗联合5-FU组小鼠MKN45细胞凋亡率最明显(P<0.05),EGFR和Bcl-2蛋白降低最显著(P<0.05)。二者表达呈正相关关系(R=0.914)。结论热疗联合5氟尿嘧啶能增加MKN45肿瘤细胞凋亡可能通过下调EGFR和Bcl-2的表达有关。

作者杨卫东张祎安琳许敏李海飞李磊郭芳朱红伟王素绢汤若琪

机构地区保定市第二医院河北大学附属医院河北医科大学

出处《贵州医药》 CAS 2016年第9期926-927,共2页 Guizhou Medical Journal

基金科研立项号:热化疗及基因治疗对胃癌抑制凋亡及血管生成的研究(16ZF039)

关键词热化疗凋亡 EGFR Bcl-2

分类号 R735.2 [医药卫生—肿瘤] R730.58 [医药卫生—肿瘤]

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