L-丝氨酸检测技术的研究进展

Development of analytical methods for L-serine

L-丝氨酸作为一种非必需氨基酸,它在药物、化工产品以及食品等方面得到了广泛应用,是一种重要的工业产物,具有很重要的研究价值。快速、准确、高通量的检测L-丝氨酸含量的方法,能够为高通量菌种选育提供坚实的基础。本文介绍了目前检测L-丝氨酸含量的多种方法,包括变色酸-分光光度法、纸层析-分光光度法、荧光猝灭法、茚三酮显色法、高效液相色谱法、酶反应检测法及毛细管电泳-电致化学发光(CE-ECL)法,同时通过比较它们的优缺点,并针对L-丝氨酸检测中存在的各种问题进行讨论分析,对L-丝氨酸的检测技术进行展望。

L-serine,as one of the nonessential amino acids,is an important industrial product with research value,widely used in medicine,chemical products and foods. As fast,accurate and high-throughput method for detecting Lserine concentration could lay the foundation for high-throughput screening of L-serine producing strains,various kinds of methods for detecting the concentration of L-serine were mainly introduced,including chromotropic acidspectrophotometry,paper chromatogram physpectrophotometer, fluorescence quenching method, ninhydrin coloration method, highperformance liquid chromatography,enzymatic assays method and CE-ECL. Moreover,the advantages and disadvantages of these methods were compared and discussed. And the development of analytical methods for L-serine was forecasted.