胶原酶活性的测定方法

On the Determination Assays for the Activity of Collagenases

胶原酶在适宜条件下可以切断天然胶原肽链。制革酶脱毛过程中胶原酶活力是影响脱毛速率和成革质量的关键。本文介绍了胶原酶活性的主要测定方法,即Rosen法、电泳法、悬浮法和FALGPA法等。其中常用的是Rosen提出的茚三酮法,茚三酮法操作和实验设备简单,测定时间短。对胶原为底物检测的特定氨基酸——羟脯氨酸,常用的是氯胺-T法,需对样品消解,实施操作较复杂。电泳法测胶原酶活性的原理稍有不同,该法只适用于定性判定或比较胶原酶的活性。

The activity of collagenases affects the depilation rate and the leather quality during enzyme unhairing due to the nature collagen could be cleaved by collagenases. The determination methods of collagenases activity were reviewed in this paper mainly including： Rosen assay, Electrophoresis assay, Suspension assay and FALGPA assay, etc. It was shown that the Rosen assay was based on ninhydrin test, which is easy operation and saves time. Chloramine-T assay was commonly used to analyze the activity of collagenase which was based on the characteristic amino acid of the collagen, hydroxyproline. However, to detect the Hyp the samples should be digested completely before the test which making the operation complex. Electrophoresis assay only could be applied to determine and compare the activity qualitatively but not quantitatively.