耐酸酵母的分离鉴定及在丢糟发酵中的应用

Separation and identification of acid proof yeast and its application in fermentation of waste fermenting grains

从白酒丢糟及酒醅中分离得到2株酵母菌YP2、YZ13,两株菌均能在pH 3.0条件下正常生长,经鉴定YP2为Candida属,与Candida pseudolambica(KM384061.1)相似度98%;YZ13为Pichia属,与Pichia kudriavzevii(LC014798.1)相似度100%.2株酵母(种子液体积比1∶1)应用于丢糟发酵中,接种量100mL/kg,在鲜丢糟870g/kg、麸皮100g/kg、硫酸铵15g/kg、生石灰15g/kg,28℃混合固态发酵60h,丢糟(干样)的真蛋白含量达172.30g/kg,比发酵前丢糟(干样)提高了28.29%,丰富了丢糟饲料蛋白,为丢糟发酵和高效饲料提供了依据.

The two yeaststrainsYP2 and YZ13 were isolated from Chinese-spirit fermenting grains and they could all grow well under the condition of pH 3.0.Strain YP2 was identified as belonging to Candida and the similarity degree of strain YP2 and Candida pseudolarnbica （KM384061.1） reached 98% ,while strain YZ13 to Pichia and the similarity degree with Pichia kudriavzevii （LC014798.1）reached 100 %.The two strains were mixed at the ratio of 1：1 in weight and applied to the fermentation of waste fermenting grains.The process of solid state fermentation lasted for 60 hours under the condition of inoculation quantity 100 mL/kg,fresh fermenting grains 870 g/kg,wheat bran 100 g/kg,ammonia sulfate 15 g/kg,quicklime 15 g/kg,temperature 28℃.The results showed that the protein content of the dry sample of waste fermenting grains reached 172.30 g/kg and increased by 28.29% and it enriched feed protein of waste fermenting grains and provided foundation for waste fermenting grains and high efficiency feed.