曼氏无针乌贼墨汁黑色素对免疫低下模型小鼠的调节作用

Immunomodulatory effects of melanin from Sepiella Maindroni ink on hypoimmune mice

探究曼氏无针乌贼墨汁黑色素(Melanin from Sepiella Maindroni Ink,MSMI)对免疫低下小鼠的调节作用。SPF级ICR小鼠60只,随机平均分为6组,其中5组(模型组,阳性对照组,墨汁黑色素低、中,高剂量组)用氢化可的松造免疫低下模型,另外一组为空白对照组。连续灌胃不同剂量的黑色素10 d,从免疫学水平评价曼氏无针乌贼墨汁黑色素的免疫调节效应。结果表明,曼氏无针乌贼墨汁黑色素(MSMI)能显著提高免疫低下小鼠的肝、脾指数(P<0.01,P<0.01),而且不同灌胃剂量的黑色素对巨噬细胞的吞噬率和吞噬能力的提高也有显著差异(P<0.05,P<0.01),曼氏无针乌贼墨汁黑色素(MSMI)也能够通过调节抗氧化体系的稳态增强机体免疫力。可见曼氏无针乌贼墨汁黑色素(MSMI)可以增强免疫器官的调节能力,尤以高剂量组最为显著。

In this study,the immunomodulatory effects of MSMI（ melanin from Sepiella maindroni ink） on hypoimmune mice induced by Hydrocortisone（ HYD） was investigated. ICR mice were selected as animal mode,which were randomly divided into six groups,including control groups,hypoimmune mice,positive control groups（ Levamisole Hydrochloride）,and MSMI treated groups of low,medium and high dosage. Except for the control group,all the other groups were treated with Hydrocortisone by subcutaneous injection.After a trial period of 10 days,the liver and spleen indexes and phagocytic capacity of macrophages were analyzed,and which were found to be significantly enhanced in the treated groups（ P〈0. 01,P〈0. 01）,（ P〈0. 05,P〈0. 01） as compared to that in the control group. Antioxidant enzyme activities were also measured and they significantly increased compared to the hypoimmune mice group.However,lipid peroxidation assayed as melondialdehyde（ MDA） reduced significantly in the treated groups compared to that in the control. It is suggested that MSMI can promote immune function of immune-depressed mice.