基于RNA-Seq技术的茶树花转录组分析

Transcriptome Analysis of Flower from Tea Plant[Camellia sinensis (L.) O. Kuntz]via RNA-Seq Technology

本研究利用RNA-seq技术对父本、母本、子代不育茶树花三个样本花的转录组进行测定,经组装分析获得403 469条高质量的Unigenes。将获得的Unigenes与SWISS-PROT、TREMBL、CDD、PFAM、NR和KOG库进行blast,共注释到307 291条Unigenes。KOG功能分类显示,有23 739个Unigenes被分为25类。KEGG通路分析表明,共识别出26 967个Unigenes涉及的pathway有328个。SSR查找发现,从403 469个Unigenes中找到46 440个含有SSR序列。这些信息为茶树不育基因筛选、不育机理研究以及分子标记开发奠定了基础。

This research established a reference transcriptome sequencing and bioinformatics analysis of male parent,female parent and offspring sterility flowers from Camellia sinensis by the RNA-Seq technology. A total of 403 469 unigenes were generated from the Camellia sinensis flowers transcriptome by using RNA-seq. A total of 307 291 unigenes were aligned to the sequences of public databases,such as Nr,Tr EMBL,Cdd,pfam and the KOG database,and 23 739 unigenes were assigned at 25 KOG classifications and 26 967 unigenes at 328 KEGG pathways. The characteristic of SSR distribution showed that 46 440 SSRs loci were detected from 403 469 unigenes. These results laid the foundation for screening the sterility gene,studying sterile mechanism and developing of molecular markers.