快速高效miRNA分析流程在黄颡鱼中的应用

Rapid and Efficient miRNA Identification Pipeline and its Application in Yellow Catfish

高通量测序技术速度快、成本低、通量高,广泛应用于miRNA领域研究。本研究基于高通量测序技术所产生的海量小RNA数据,结合已有的数据分析软件,开发了一套快速高效一键化的miRNA分析流程。该流程整合多个生物信息学数据分析软件,对多个miRNA高通量测序数据集进行标记、整合和去冗余分析,只需运行一次核心程序就可以实现对多个miRNA高通量测序数据的分析,避免每个样本单独数据分析的技术重复,精简后的数据集能大幅度减少软件计算量,显著提高软件运行效率。本研究利用快速高效miRNA分析流程分析黄颡鱼性腺XX卵巢、XY精巢、YY精巢的miRNA高通量测序数据,获得一批准确的黄颡鱼保守miRNA。在相同参数设置下,miRNA分析流程可以显著节约分析时间。该流程最终输出结果为多样本整合后的miRNA表达数据,便于研究者直接进行样本之间的比较和miRNA的表达差异,减少研究者手动整合分析结果的操作步骤。miRNA分析流程针对多样本miRNA测序数据具有明显的优势,样本越多测序量越大,软件运行效率越高。针对日益积累的海量小RNA测序数据,miRNA分析流程高效快速一键化数据处理优势将会越来越明显。

High-throughput sequencing technologies have been widely used in miRNA studies with the advantages of high speed, low cost and high throughput. Based on the massive miRNA data from high-throughput sequencing, we developed a rapid and efficient miRNA identification pipeline integrated with several data analysis softwares. By marking, merging and clustering of several miRNA sequencing data sets, our pipeline can avoid duplicate analysis processes for every single data set, which can greatly reduce the amount of calculation and significantly improve the software efficiency. Just run one more time of our pipeline, all the samples will be analyzed. In this study, we analyzed miRNA sequencing data from XX ovaries, XY testis and YY testis of yellow catfish（Pelteobagrus fulvidraco）with this pipeline, and identified a number of conserved miRNAs, with significant reduce in time in the same parameters by pipeline. The integrated results from our pipeline are comparable between samples and easily to do further miRNA differentially expression analysis, which will greatly reduce the manual integration operation for every single results. Our miRNA identification pipeline has obvious advantages in processing multiple data sets. The more samples and greater data sets, the higher the efficiency. The advantages of our pipeline will be more and more obvious with the increasing accumulated miRNA sequencing data.