贵阳市食品中沙门氏菌污染检测

Detection of Salmonella spp.in foods from Guiyang city by PCR

利用沙门氏菌inv A基因序列设计引物并建立PCR反应,该反应灵敏度为3.6×102CFU/m L。在1m L样中含菌量106CFU/m L时增菌4h、含菌量103CFU/m L时增菌8h、含菌量102CFU/m L时增菌12h后可检出。从贵阳市各区随机购买113份肉、蛋类食品,检出10份样品含有沙门氏菌,总检出率为8.8%。该方法准确、灵敏、快捷、成本低,可广泛应用于沙门氏菌检测。

In this study, we designed a pair of primers specific to invA gene of Salmonella, and estab- lished PCR reaction conditions. The reaction sensitivity is 3.6 × 10^2CFU/mL. In one mL sample, 106 CFU/ml, 10^3 CFU/ml and 10^2 CFU/ml can be detected after 4h, 8h and 12h enrichment with LB cul- ture media respectively. A total of 89 meat and 24 egg samples from supermarket or trade market in Guiyang city were analyzed, ten samples contained Salmonella, and the contamination rate was 8.8%. Our method is accurate, sensitive, fast and with low cost, hence it can be widely used to detect Salmo- nella in foods.