ERα特异性抑制剂对KM小鼠植入前胚体外发育的影响

The influence of ERα-specific antagonist on KM mouse preimplantation embryos development in vitro

目的探讨ERα在KM小鼠植入前胚体外发育中的作用。方法以空白M16培养液为对照组,观察不同浓度的ER抑制剂(ICI182780)、ERα特异性抑制剂(MPP)、ERβ特异性抑制剂(PHTPP)和ERα特异性抗体对KM小鼠1-细胞胚体外发育至4-细胞胚和囊胚的影响。结果与M16对照组比较,0.5和1μmol/L的ICI182780、10μmol/L的MPP明显降低4-细胞胚的发育率,导致囊胚发育失败(P<0.01);25和50μmol/L的MPP、1μg/m L的ERα特异性抗体将1-细胞胚阻滞在2-细胞阶段,并且50μmol/L的MPP对细胞有毒性作用(P<0.01);0.5μg/m L的ERα特异性抗体使细胞丧失发育至囊胚的能力(P<0.01);各浓度的PHTPP均未被观察到有上述作用(P>0.05)。结论 ER抑制剂、ERα特异性抑制剂和ERα特异性抗体均能抑制小鼠1-细胞胚的体外发育。

Objective The objective of this study was to determine whether ERα plays a role in KM mouse preimplantation embryos development in vitro. Methods With blank M16 cultures as the control group,we observed the effects of different concentrations of antiestrogen ICI182780,ERα- specific antagonist MPP,ERβ- specific antagonist PHTPP and ERα specific antibody on the development of KM 1- cell embryos to 4- cell and blastocysts in vitro. Results Compared with blank M16 culture group,0. 5 and 1 μmol / l concentrations of ICI182780,as well as 10 μmol / l concentration of MPP,had significantly decreased the developmental rate of 4- cell embryos and lead to come to nothing of blastocysts（ P〈 0. 01）. And,25 and 50 μmol / l concentrations of MPP,as well as 1 μg / ml concentration of ERα specific antibody,had blocked 1- cell embryos at the 2- cell stage. Meanwhile,50 μmol / l concentration of MPP had a toxic effect on embryos（ P〈 0. 01）. 0. 5 μg / ml concentration of ERα specific antibody had made the embryos lose the ability of development to the blastocysts（ P〈 0. 01）. Finally,any concentration of PHTPP had been observed to have not any effect on the development of preimplantation embryos in vitro（ P〈 0. 05）. Conclusion Antiestrogen and ERα- specific antagonist,as well as ERα specific antibody,could inhibit the development of 1- cell embryos in vitro.