无果枸杞芽提取物对低密度脂蛋白受体^(-/-)小鼠的抗动脉粥样硬化作用研究

Effect of fruitless lyceum-sprout extracts on atherosclerosis in LDLR^(-/-) mice

目的探讨无果枸杞芽提取物(fruitless lycium-sprout extracts,FLE)对低密度脂蛋白受体(LDLR)-/-小鼠动脉粥样硬化病程的影响,为开发FLE调控血脂、抑制动脉粥样硬化病程进展的药用价值提供理论依据。方法选择12周龄雄性LDLR-/-小鼠48只,分为正常饮食组16只,动脉粥样硬化模型组(模型组)16只,FLE组16只。小鼠体质量在研究开始时测量,每4周测量1次,分别于12、16、20和24周时检测,测量体质量当天采集血液,分离血清,检测TC、TG及氧化型低密度脂蛋白(oxLDL)/β2糖蛋白I(β2GPI);收集小鼠尿液,试剂盒测定尿液中11-脱氢血栓素B2(11-dhTXB2)含量。24周时用组织化学染色法观察各组小鼠主动脉斑块形成情况,评估FLE的疗效。结果模型组小鼠16、20、24周时血清TC、TG和尿11-dhTXB2水平明显高于FLE组(P<0.05,P<0.01)。模型组小鼠20、24周时oxLDL/β2GPI复合物水平明显高于FLE组,差异有统计学意义(P<0.05,P<0.01)。正常饮食组、模型组和FLE组小鼠24周时动脉斑块面积分别为(3.36±0.87)%、(20.71±2.17)%和(6.16±0.76)%。正常饮食组几乎看不到斑块形成,模型组斑块明显增加,而FLE组斑块面积较模型组明显受到抑制(P<0.01)。结论 FLE能有效抑制高脂饮食诱发的小鼠动脉斑块形成,延缓病程发展。

Objective To study the effect of fruitless lyceum-sprout extracts（FLE）on atherosclerosis in LDLR^-/-mice.Methods Forty-eight male LDLR^-/-mice aged 12 weeks were randomly divided into normal diet（ND）group（n=16）,atherosclerosis model group（n=16）and FLE group（n=16）.Their BMI was measured every 4weeks.Their serum TC,TG,oxLDL/β2GPI levels and urinary 11-dhTXB2 level were measured.Formation of atherosclerotic plaques in aorta was detected with histochemiscal staining.The effect of FLE on atherosclerosis was assessed.Results The serum TC,TG,oxLDL/β2GPI levels and urinary 11-dhTXB2 level were significantly higher in atherosclerosis model group than in FLE group at weeks 16,20 and 24（P〈0.05,P〈0.01）.The oxLDL/β2GPI level was significantly higher in atherosclerosis model group than in FLE group at weeks 20 and 24（P〈0.05,P〈0.01）.The size of atherosclerotic plaques was 3.36%±0.87%,20.71%±2.17% and 6.16%±0.76%respectively in ND group,atherosclerosis model group and FLE group at week 24.However,atherosclerotic plaque was hardly detected in ND group.The size of plaques was significantly larger in atherosclerosis model group than in FLE group（P〈0.01）.Conclusion FLE can effectively inhibit the formation of atherosclerotic plaques and delay its progession in rats fed with high fat diet.