ROR-γt、Foxp3在前列腺增生组织中的表达及意义

Expressions and significances of ROR-γt and Foxp3 in benign prostatic hyperplasia

目的 探讨维甲酸相关孤儿受体（ROR-γt）、叉状/翼状螺旋转录因子（Foxp3）在前列腺增生（BPH）组织中的表达及临床意义。方法 选取2013年5～12月在本院行经尿道前列腺电切术（TURP）的BPH患者80例作为实验组（根据合并有和无组织学炎症分为实验组1和实验组2）,另取健康男性18～27岁因外伤意外死亡法医及时尸体解剖并伦理委员会同意的5例前列腺组织作为对照组,迅速取出相应前列腺组织保存于液氮中,运用实时荧光定量PCR（QRT-PCR）检测ROR-γt、Foxp3mRNA的相对表达量,对两组研究对象的ROR-γt与Foxp3关系进行分析。结果 实验组1的ROR-γt mRNA含量明显高于对照组和实验组2（P〈0.05）;实验组1的Foxp3mRNA含量明显低于对照组和实验组2（P〈0.05）;ROR-γt、Foxp3mRNA在伴有组织炎症的前列腺增生组织与无组织学炎症的BPH组织中表达均有相关（r=0.623,P=0.002;r=-0.588,P=0.006）。结论 组织学炎症在BPH生组织中发挥重要作用,ROR-γt介导炎症反应、Foxp3抑制组织学炎症,其共同作用使组织局部微环境中Th17/Treg比例失衡,从而可能导致BPH的发生。

Objective To study the expressions and clinical significances of ROR-γt and Foxp3 in benign prostatic hyperplasia. Methods A total of 80 benign prostatic hyperplasia （BPH） cases treated with transurethral resection of the prostate （TURP） during May and Dec. 2013 were selected as the experimental group, which were subdivided into test group 1 （with inflammation） and test group 2 （without inflammation）, and 5 cases of healthy male aged 18-27 years who died of trauma were involved as the control group. ROR-γt and Foxp3 mRNA relative expressions were detected with QRT-PCR, and their relationship was analyzed. Results ROR-γt mRNA content was significantly higher in test group 1 than in the control group and test group 2 （P〈0.05）. Foxp 3 mRNA content was significantly lower in test group 1 than in the control group and experimental group 2 （P〈0.05）. ROR-γt and Foxp 3 mRNA were associated with BPH with inflammation and BPH without inflammation （R = 0. 623, P = 0. 002 ; R =- 0. 588, P = 0. 006）. Conclusion Inflammation plays an important role in BPH. ROR-γt mediates inflammation while Foxp3 inhibits inflammation, causing the imbalance of Th17/Treg ratio in the local microenvironment, which leads to the occurrence of prostate hyperplasia.