PKGⅡ对乳腺癌MCF-7细胞株中抑癌基因蛋白表达的影响

Effect of type Ⅱ cGMP-dependent protein kinase on tumor suppressors in breast cancer cell line MCF-7

目的:研究乳腺癌MCF-7细胞株中PKGⅡ对抑癌基因PTEN、p21、p53、BRCA1以及BRCA2蛋白表达的影响。方法:用编码PKGⅡc DNA的腺病毒载体感染MCF-7细胞,使其高表达PKGⅡ并以相应激活剂8-p CPT-cGMP激活;蛋白质印迹法检测MCF-7细胞p53、p21、PTEN、BRCA1以及BRCA2因子表达量。结果:PKGⅡ表达增高并激活后可使乳腺癌MCF-7细胞株中抑癌基因的蛋白表达降低。结论:PKGⅡ通过降低癌细胞的活动水平从而降低抑癌基因蛋白的表达。

Objective： To investigate the effects of PKGⅡ on tumor suppressor genes including PTEN, p21, p53 ,breast cancer type 1 susceptibility protein （BRCA1） and BRCA2 in breast cancer cell line MCF- 7. Methods ： MCF-7 cells were transfected with adenoviral vector encoding cDNA of PKG Ⅱ and the kina- ses were activated by respective activator. Western blotting was applied to detect the influence of PKG Ⅱ on the expression of tumor suppressor proteins, including p53, p21, FFEN, BRCA1 and BRCA2. Results： When PKGⅡ expression and activity were increased, the expression of PTEN, p21, p53, BRCA1 and BRCA2 was decreased. Conclusion： PKG Ⅱ could inhibit the expression of PTEN, p21, p53, BRCA1 and BRCA2.