广西不同产地瑶药猛老虎中白花丹醌的含量比较研究

Comparison Study on the Plumbagin Content in Plumbago Zeylanica Collected from Different Areas of Guangxi

目的:比较广西不同产地瑶药猛老虎中的白花丹醌的含量,为猛老虎药材的合理开发利用提供依据。方法:采用HPLC法,采用ZORBAX Eclipse XDB C_(18)(4.6 mm×250 mm,5μm)色谱柱,流动相为甲醇∶水(60∶40,V:V),检测波长为265 nm,流速1 m L/min。结果:白花丹醌在0.104μg^2.08μg范围内线性关系良好,相关系数r^2=0.9999,回收率范围为98.05%~100.52%,RSD%为1.12%。12个产地16个批次的猛老虎药材中白花丹醌的含量范围为0.004%~0.311%。结论:不同产地的瑶药猛老虎中的白花丹醌含量存在一定的差异,采用高效液相色谱法可以对猛老虎药材进行有效的质量控制。

Objective： To compare plumbagin content in Plumbago zeylanica from different areas of Guangxi, in order to provide references for appropriate utilization and development of Plumbago zeylanica （A Yao medicine named Menglaohu） medicinal materials. Methods： The content of plumbagin from Plumbago zeylanica was determined by HPLC. The analysis was carried out on a ZORBAX Eclipse XDB C18 column （4.6 mm×250 mm, 5 μm）. The mobile phase was consisted of methanol： water （60：40, V：V） in isocratic elution with flow rate 1.0 mL/ min and the detection wave length was 265 nm. Results： The plnmbagin showed good linearity （r2=0.9999） in a relatively wide concentration range （0.104μg-2.08μg）. The recovery rate of the method was in the range of 98. 05%-100.52%, and the RSD was 1.12%. The plumbagin content range of 16 batches of Plumbago zeylanica from 12 places of Guangxi was 0.004%-0.311%. Conclusion： Plumbagin contents in Plumbago zeylanica from different areas of Guangxi had certain differences and the HPLC method can be used to control the quality of the Plumbago zeylanica medicinal materials.