不同月龄马身猪和大白猪血清生长激素及其受体的变化规律

Study on the Change Trend of Growth Hormone(GH)and Growth Hormone Receptor(GHR)in Serum at Different Months in Mashen and Large White Pigs

试验旨在探讨马身猪和大白猪血清中生长激素（growth hormone,GH）及其受体（growth hormone receptor,GHR）在生长发育过程中的变化规律,采用ELISA方法检测马身猪和大白猪0～6月龄血清中GH和GHR的浓度。结果表明,马身猪和大白猪在0～6月龄血清中GH浓度变化趋势基本相似,随着日龄的增长,血清中GH浓度呈逐渐上升的趋势,分别在4和5月龄时达到最高值,随后又逐渐降低,但与峰值无显著差异（P〉0.05）,在3和4月龄时,马身猪血清中GH浓度略高于大白猪,而在其他月龄,大白猪血清中GH均高于马身猪;0～6月龄,马身猪血清中GHR浓度无显著差异（P〉0.05）,大白猪血清中GHR的含量在1月龄时最低,与4月龄差异极显著（P〈0.01）,与6月龄差异显著（P〈0.05）,其他月龄间差异不显著（P〉0.05）,0～2月龄,马身猪血清中GHR含量高于大白猪,但只有在1月龄时差异达极显著水平（P〈0.01）;3～6月龄,大白猪血清中GHR含量高于马身猪,且在4和6月龄时差异显著（P〈0.05）,5月龄时差异极显著（P〈0.01）。血清中GH和GHR浓度与猪的发育阶段和遗传背景有关,其变化规律与猪生长速度的变化趋势相一致。

The purpose of this study was to understand the change trend of growth hormone（GH）and growth hormone receptor（GHR）in serum of Mashen and Large White pigs during the period from 0to6 months of age,and to analyze the influence of GH and GHR on growth rate.The method of ELISA was used to detect the concentration of GH and GHR in serum of Mashen and Large White pigs from 0to 6months of age.The results showed that the variation trends of GH in Mashen pig was roughly the same as in Large White pig,the GH concentration was increased with age increasing after birth and reached the peaks at 4and 5months of age for Mashen and Large White pigs,respectively,and then decreased gradually.The serum GH concentration in Mashen pig was a little greater than that in Large White pig at3 and 4months of age,on the contrary,the serum GH concentration in Large White pig was greater than that in Mashen pig at other months.During the period from 0to 6months of age,the difference of GHR concentration in serum was not significant in Mashen pig（P〉0.05）.In Large White pig,the serum GHR concentration at 1 month of age was lowest,and was significantly lower than that at 4and 6months of age（P〈0.01;P〈0.05）.During the period of 0to 2months of age,the GHR concentration in Mashen pig was greater than that in Large White pig,but the difference was extremely significant only at1 month of age（P〈0.01）.Conversely,the serum GHR concentration in Large White pig was greater than those in Mashen pig during the period from 3to 6month of age,there was significant difference at 4and 6months of age（P〈0.05）,and there was extremely significant at 5months of age（P〈0.01）.The concentration of GH and GHR in serum was related to the developmental stages and genetic background of pig,and its change trend was in accordance with the trend of growth rate.