鸡传染性法氏囊病病毒BJQ902株在DF-1细胞系上增殖工艺研究

Study on Proliferation Technology of Chicken Infectious Bursal Disease Virus BJQ902 Strain in DF-1 Cell Line

试验旨在通过DF-1细胞增殖获得高效价的鸡传染性法氏囊病病毒（infectious bursal disease virus,IBDV）抗原。通过病毒接种量、收毒时间、接毒时间、温度和维持液血清浓度5个培养条件的筛选和优化,对IBDV BJQ902株在DF-1细胞上的增殖工艺进行了研究。结果表明,IBDV BJQ902株在DF-1细胞上最佳增殖条件为：在37℃条件下培养,接毒量在0.01%～0.1%之间,接种时间为细胞传代后生长48～72h,维持液血清浓度为1%～2%,收毒时间为病毒接种后60～72h。在此培养条件下增殖病毒毒价在108.3～108.7 TCID50/0.1mL之间。

In order to produce high titers of infectious bursal disease virus（IBDV）antigen,the proliferation technology of chicken IBDV BJQ902 strain in DF-1cell line was studied by the selection and optimization of the following five culture conditions,including the amount of inoculated virus,harvest time,inoculation time,culture temperature and serum concentration in maintenance media.The results showed that the optimal proliferation conditions of IBDV BJQ902 strain in DF-1cell line were obtained as follow：The culture temperature was 37℃,the inoculum concentration was between 0.01% and 0.1%（V/V）,the inoculation time was between 48 and 72hafter cell passage,the serum concentration in maintenance media was between 1%and 2%,the harvest time was between 60 and 72hafter inoculation.Under the optimal conditions,the virus titers were between 108.3 and 108.7 TCID50/0.1mL.