微波加热快速提取芽孢杆菌与乳酸菌DNA

Rapid Extraction of Bacillus and Lactobacillus DNA by Microwave Heating

试验旨在研究快速、简单、高效提取芽孢杆菌与乳酸菌DNA的方法。在微波炉额定功率800 W下,对芽孢杆菌和乳酸菌进行微波处理40～150s,离心获取上清,收获细菌DNA,将样本DNA PCR扩增后进行琼脂糖凝胶电泳验证,测定其纯度和产量后测序。结果显示,在微波炉功率800W条件下加热40～150s均可有效提取芽孢杆菌与乳酸菌DNA。所得DNA质量较好（D_（260nm）/D_（280nm）在1.8～2.1之间）。DNA浓度符合PCR检测要求,目的条带清晰,所得测序结果满足常规分子生物学研究。该微波提取方法具有简单、快速、高效的特点,且具有广泛的实用性,为乳酸菌与芽孢杆菌快速分子检测提供了简便手段。

The assay was aimed to investigate a fast,simple and efficient method with microwave heating for extracting DNA of Bacillus and Lactobacillus.Treated in a microwave oven at rated power（800W）for a certain time（40to 150s）,then centrifuged for supernatant.The supernatant was transferred to a sterile tube for PCR testing and agarose gel electrophoresis.Sequencing was carried out after determining the purity and yield.The results showed that heating for 40 to 150s at 800 W microwave power conditions could effectively extract DNA of Bacillus and Lactobacillus.The obtained DNA quality was good（D_（260nm）/D_（280nm） was 1.8to 2.1）.The DNA concentration in line with the PCR testing requirements;Sequencing results was satisfied with the routine molecular biological research.The microwave extraction method had simple,fast and efficient features,and a wide range of practical,which provided a simple means for rapid molecular assay.