喉癌中高危型HPV、ATM表达及临床意义

Expression of high-risk HPV and ATM in human laryngeal squamous cell carcinoma and its clinical significance

目的:通过检测喉癌患者肿瘤组织中ATM及HPV的表达,分析其与预后的关系。以探讨ATM,HPV在喉癌发生发展中的作用。方法:采用PCR法检测63例肿瘤组织和63例癌旁周围正常喉组织HPV16/18表达情况,免疫组化法检测63例肿瘤组织和30例癌旁周围正常喉组织的ATM蛋白的表达。Kaplan-Meier法行生存分析。结果 :63例喉鳞癌组织中,HPV16/18阳性率为39.7%,癌旁组织HPV16/18阳性率为9.5%,差异具有统计学意义(P<0.05)。ATM蛋白在喉癌和癌旁组织中的阳性表达率分别为41.3%和83.3%,差异具有统计学意义(P<0.05)。HPV16/18与ATM表达之间无相关性。喉癌HPV16/18阳性组5年生存率为56.0%,阴性组5年生存率为52.6%,差异无统计学意义(P>0.05);63例喉癌ATM蛋白表达阳性组的5年生存率为65.4%,阴性组5年生存率为45.9%,差异无统计学意义(P>0.05)。结论:喉癌中HPV16/18、ATM蛋白表达异常,HPV与ATM蛋白表达无相关性且两者表达与喉癌预后无关。

Objective To study the expression of human papillomavirus （HPV） and ATM protein in laryngeal squamous cell carcinoma （LSCC）, and discuss the correlation among the expression and its prognosis Method The expression of HPV16/18 mRNA was detected by PCR, and the expression of ATM proteins by immunohistochemical method in 63 LSCC specimens and 30 specimens of normal tissue adjacent to cancer. Results The positive expression rates of HPV16 /18 and ATM protein in LSCC group were 39.7% （25 /63） and 41. 3% （26 /63） respectively and those of HPV16 /18 and ATM protein in normal group were 9.5% （ 6/ 63 ） and 83.3% （25 /30） respectively. There was no correlation between the expression of ATM and HPV16/18 in LSCC. The accumulative 5-year survival rates of HPV16/18 positive group and HPVI6/18 negative group in 63 patients were 69.8% and 52.6% respectively,and there was no significant difference （P〉 0.05. The aecumulative 5-year survival rates of ATM positive group and ATM negative group in 63 patients were 65.4% and 45.9% respectively and there was no significant difference （P〉0.05. Conclusion Both HPV16/18 and ATM are abnormally expressed in LSCC, but there is no correlation between the expression of HPV16/18 and ATM and the expression and its prognosis.