儿童急性下呼吸道人博卡病毒感染血清特异性抗体检测的灵敏度与特异度分析

Sensitivity and specificity of detection of serum specific antibody for children with acute lower respiratory Boka virus infection

目的研究血清特异性抗体检测对儿童急性下呼吸道人博卡病毒(HBoV)感染的灵敏度与特异度,为儿童急性HBov感染诊断提供依据。方法选择2010年7月-2015年12月在医院接受治疗的急性下呼吸道人博卡病毒感染患儿140例,另选择健康体检儿童50名,对所有受试者血清特异性抗体及痰液常规检测结果进行比较。结果血清检测患儿阳性率为52.63%,高于痰液检测阳性率44.74%,血清检测的灵敏度、特异性及准确度均明显高于痰液检测,差异均有统计学意义(P<0.05);血清检测阳性患儿中表现为喘息、咳嗽、发热以及鼻塞与流涕的患儿比例均显著高于痰液检测的患儿比例,差异均有统计学意义(P<0.05);血清检测阳性患儿中临床诊断为支气管肺炎的比例高于痰液检测的比例,差异有统计学意义(P<0.05);血清与痰液检测博卡病毒、支原体属、合胞病毒以及鼻病毒等分布构成比,差异均无统计学意义。结论血清特异性检测儿童急性下呼吸道HBoV感染具有较高的灵敏度与特异度,并且检测结果与临床表现、临床诊断符合程度很高。

OBJECTIVE To explore sensitivity and specificity of detection of serum specific antibody for children with acute lower respiratory Boka virus （HBoV） infection, so as to provide the basis for diagnosis of acute HBov infection in children. METHODS A total of 140 patients of children with acute lower respiratory HBoV infection disease from Jul. 2010 to Dec. 2015 in our hospital were selected, and another 50 cases of healthy volunteers were also selected. The serum specific antibody and sputum test results for all subjects were compared. RESULTS The positive rate of serum detection was 52.6%, which was higher than 44.74% of the sputum detection. The sensi- tivity, specificity and accuracy of the serum detection were significantly higher than those of the sputum detection （P〈0.05）. The ratios of wheezing, cough, fever and nasal congestion and runny nose in positive patients of the serum detection were significantly higher than those of the sputum detection（P〈0.05）. The percentage of clinical diagnosis of bronchial pneumonia of the serum detection was higher than that of the sputum detection（P〈0.05） and the constituent ratios of Boca viruses, mycoplasma, syncytial virus and rhinovirus of the serum detection and sputum detection had no significance. CONCLUSION Serum specific detection of HBoV infection in children with acute lower respiratory tract infection has a high sensitivity and specificity, and the coincidence degree of the detec- tion results with clinical manifestations and clinical diagnosis is very high.