零余子粗多糖超声波辅助提取工艺优化

Optimization on ultrasound-assisted extraction process of crude polysaccharide from bulbil

利用响应面法优化零余子粗多糖的超声波辅助提取工艺。通过单因素试验考察液料比、超声功率与提取时间对粗多糖得率的影响。在单因素试验的基础上,采用BoxBehnken中心组合方法设计试验,以粗多糖得率为响应值进行响应面分析,获得最优提取工艺为料液比18∶1(mL/g)、超声功率760 W、提取时间23 min,粗多糖得率可达60.68%,与预测值相对误差为2.03%,结果表明预测值与试验值之间具有良好的拟合度,采用响应面法对零余子多糖超声波提取条件进行优化合理可行。

Response surface analysis was proposed to optimize the ul trasound-assisted extraction process of crude polysaccharides from bulbils. The effects of solid-liquid ratio, ultrasonic power and extrac- tion time on crude polysaccharide yield were investigated by single factor experiments. Response surface analysis was carried out by using the crude polysaccharide yield as response value and the box Behnken method to design the experiments. The optimum extraction conditions were obtained as follows： solid liquid ratio, 18： 1 （mL/g）, ultrasonic power, 760 W; extraction time, 23 rain and the crude polysaccharides yield could be up to 60.68%, which agreed with the predicted value （the relative error is 2.03%）. The results demonstrated that it is reasonable and practicable to optimize the ul- trasonic extraction process using response surface methodology.