摘要
目的探讨HBV相关性和非HBV相关性肝细胞癌(HCC)组织的长链非编码RNA(lncRNA)的表达情况。方法选择行手术治疗的HBV相关HCC患者42例、非HBV相关HCC患者45例,采用原位杂交技术检测两组患者肿瘤维修组和癌旁组织中肌动蛋白丝相关蛋白1-反义RNA1(AFAP1-AS1)、肝癌微血管浸润相关lncRNA(MVIH)和肺腺癌转移相关转录本1(MALAT-1)的表达水平,并比较不同临床特征HCC患者肿瘤组织AFAP1-AS1、MVIH和MALAT-1的表达情况。结果在两组患者中,肿瘤组织AFAP-AS1的染色分数低于癌旁组织(P<0.05),而肿瘤组织MVIH和MALAT-1的染色分数高于癌旁组织(P均<0.05)。HBV相关性HCC组癌旁组织中的AFAP-AS1染色分数高于非HBV相关HCC组癌旁组织(P<0.05),而两组肿瘤组织AFAP-AS1染色分数、两组肿瘤组织或癌旁组织中MVIH和MALAT-1的染色分数比较,差异均无统计学意义(P均>0.05)。在HBV相关性HCC、非HBV相关性HCC患者中,AFAP1-AS1、MVIH和MALAT-1在不同的分期和分型患者的肿瘤组织中的表达水平比较,差异均无统计学意义(P均>0.05)。结论在HCC组织中AFAP1-AS1表达下调而MVIH和MALAT-1表达上调,三者均参与了HCC的发生发展,而AFAP1-AS1的表达可能还受到HBV相关生物学过程的调节。
Objective To explore the expressions of long non-coding RNAs( lncRNA) in HBV-related and non-HBV-related hepatocellular carcinoma( HCC) tissues. Methods Forty-two patients with HBV-related and 45 with non-HBV-related HCC undergoing surgery were enrolled. in situ hybridization technique was used to detect the expression levels of actin filament-associated protein 1-antisense RNA1( AFAP1-AS1),lncRNA associated with microvascular invasion in HCC( MVIH) and metastasis-associated lung adenocarcinoma transcript 1( MALAT-1) in tumor tissues and paracarcinoma tissues of both groups. And the expressions of AFAP1-AS1,MVIH and MALAT-1 in the tumor tissues were compared between the HCC patients with different clinical features. Results In the two groups,the staining fraction of AFAP-AS1 was lower but the staining fractions of MVIH and MALAT-1 were higher in the tumor tissues compared to the paracarcinoma tissues( all P〈0. 05). The staining fraction of AFAP-AS1 in the paracarcinoma tissues of HBV-related HCC group was higher than that in the paracarcinoma tissue of non-HBV-related HCC( P〈0. 05),but no significant difference in the staining fraction of AFAP-AS1 in the tumor tissues was found between the two groups( all P〉0. 05). And there were no significant differences in the staining fractions of MVIH and MALAT-1 in either tumor tissues or paracarcinama tissues between the two groups( all P〉0. 05). For the patients with HBV-related HCC or non-HBV-related HCC,no significant differences in the staining fractions of AFAP-AS1,MVIH and MALAT-1 in the tumor tissues were found between the patients with different stages or classifications( all P〉0. 05). Conclusion The expression of AFAP1-AS1 decreases but the expressions of MVIH and MALAT1 increase in HCC tissues,and all of them are involved in the development of HCC. And the expression of AFAP1-AS1 may also be regulated by HBV-related biological processes.
出处
《广西医学》
CAS
2016年第10期1385-1388,共4页
Guangxi Medical Journal