安息香配伍冰片在人工胃肠液中的成分变化研究

Research on composition change of Anxixiang after compatibility with Bingpian in artificial gastric and intestinal fluid

目的:研究安息香与冰片配伍后其主要成分苯甲酸及总香脂酸在人工胃肠液中的含量变化情况。方法:采用高效液相色谱法(HPLC)研究苯甲酸含量变化,采用紫外分光光度法(UV)研究总香脂酸含量变化。结果:HPLC测定苯甲酸含量大小为:人工肠液>水>人工胃液,配伍冰片组含量大于单味样品组含量;UV测定总香脂酸含量大小为:人工肠液>水>人工胃液;配伍冰片组含量大于单味样品组含量。结论:HPLC和UV测定结果表明安息香在人工肠液中吸收更好,而在人工胃液中吸收较少,表明安息香主要在肠道代谢;与冰片配伍有利于安息香的吸收,体现了冰片与安息香相须相使的作用,表明了配伍的合理性。

Objective： To investigate the content change of main ingredients of Anxixiang （benzoic acid and total balsam acid） after compatibility with Bingpian in artificial gastric and intestinal fluid. Method： High performance liquid chromatography （HPLC） was used to detect benzoic acid content changes. UV spectrophotometry （UV） was used to research balsam acid content changes. Result： HPLC determination results showed that benzoic acid content in artificial intestinal fluid was larger than that in water which was more than in artificial gastric fluid. Bingpian increased benzoic acid content in each fluid. UV determination showed that total balsam content in artificial intestinal fluid was larger than that in water which was more than that in artificial gastric fluid. Bingpian increased total balsam content in each fluid. Conclusion： The HPLC and UV results indicate that Anxixiang absorption in artificial intestinal fluid is better than in artificial gastric fluid which may be caused by intestinal metabolism. Compatibility of Bingpin increases the absorption of Anxixiang which reflects rationality of compatibility.