尿中氟化物批量检测的质量控制的应用

A exploration and application of a quality control method for urinary fluoride detection

目的 分析尿中氟化物批量检测中实施的质量控制措施效果,为检测活动提供质控经验.方法 检测尿样从采集到检测全程采取统一管理和质控的方式,按照《尿中氟化物的测定离子选择电极法》（WS/T 89-1996）采取人员集中方式进行检测.在检测过程中实施空白实验、标准物质检测、人员比对、平行样检测、仪器比对及留样再检测等内部质量控制措施.结果 空白实验检测结果均低于最低检出限;标准物质检测结果在标准值参考范围内,标准物质检测平均值与参考值比较差异无统计学意义（t=0.01、0.00、0.02,P均＞ 0.05）,｜Z｜值均＜1;人员比对及平行样检测结果比较差异均无统计学意义（P均＞0.05）;仪器比对检测结果差异无统计学意义（t=0.129,P＞0.05）;留样复检结果差异无统计学意义（t=-0.230,P＞ 0.05）.检测尿样9 123份,尿氟几何均数为0.58 mg/L.结论 大规模尿中氟化物样品检测应在样品采集前准备、采集、运输、检测、结果出具等不同阶段全程实施不同质量控制,控制器材、人员、仪器、试剂等引起的误差,保证检测结果质量受控.

Objective To analyze the quality control measures on urinary fluoride testing in the implementation,and to provide experience in quality control for testing activities.Method According to the ＂Determination of Fluoride in Urine-Ion Selective Electrode Method ＂（WS/T 89-1996）,focusing on ways to complete testing all the samples in a short time,implementing internal quality control measures in the testing before,during and after the implementation by different ways as blank experiment,standard substance detection,personnel parallel experiment,paralleled detection,instrument comparison and sample test repeat,were carried out.Results The test results of blank experiment were lower than the lowest detection limit;the test results of standard material were within the scope of the standard reference,there was no significant difference between the mean value and the reference value （t =0.01,0.00,0.02,all P 〉 0.05）,｜Z｜ values were all less than 1;the results of personnel parallel experiment,paralleled detection,instrument comparison test results and sample test repeat were not significantly different statistically （all P 〉 0.05）;the results of instrument comparison and sample test repeat were not significantly different statistically （t =0.129,0.034,all P 〉 0.05）.A total of 9 123 urine samples were tested,the geometric mean of urinery fluoride was 0.58 mg/L.Conclusion Different quality control measures should be implemented through all the testing,control personnel errors,instruments,and reagents,etc,to ensure the quality of the entire test results.