摘要
目的探讨5-羟色胺转运蛋白基因5-HTFLPR及STin2与抑郁症临床症状的关联性。方法研究共纳入401名中国汉族抑郁症患者,提取外周血基因组DNA,采用PCR分别对5-HTTLPR及STin2进行基因多态性分析;同时对每位患者进行HAMD-17及HAMA量表评估;然后进行关联性分析。结果(1)5-HTfLPR各基因型患者发病年龄分布差异有统计学意义(F=3.281,P=0.039),L/S组患者发病年龄最小,I/L组患者发病年龄最大。同时三组基因型患者的HAMA1条目(焦虑心境)得分差异具有统计学意义(P=0.010),其中L/S组患者得分[(2.34±0.80)分]显著高于S/S组患者[(2.08±0.85)分,校正P=0.018]。(2)STin2各基因型患者的HAMD10(精神性焦虑)、HAMA1(焦虑心境)、HAMA3(害怕)条目及精神性焦虑因子评分差异均具有统计学意义(P值分别为:O.014、0.044、0.036及0.006),其中12/10基因型组患者得分最高[分别为:(2.40±0.83)分、(2.38±0.90)分、(1.42±1.04)分、(14.60±4.26)分)],与12/12组患者相比,HAMD10(精神性焦虑)条目[(2.11±0.77)分]及精神性焦虑因子得分[(12.96±3.78)分]均差异具有统计学意义(校正JP值分别为:0.018、0.006)。结论在中国汉族抑郁症人群中,5-HTTLPR与抑郁症发病年龄相关;5-HTFLPR及STin2两种基因多态性均与患者焦虑样症状表型存在关联,杂合子患者的焦虑症状更为突出,为抑郁症临床分型及优化治疗提供了一定线索。
Objective To determine the potential correlation of 5-HTT gene polymorphisms ( 5-HT- TLPR and STin2) with clinical manifestations in depression. Methods A total of 401 depressed patients, all of Chinese Han region ,were collected and genotyped by polymerase chain reactions (PCR). All patients were evaluated using a 17-item Hamilton depression rating scale (HAMD-17) and Hamihon anxiety rating scale (HAMA) , and then associated analysis was applied. Results ( 1 ) The age of onset in patients with L/S genotype of 5-HTLPR polymorphism were much younger than that of patients with L/L and S/S genotype (F= 3.281, P= 0.039). Besides, there was also a significant difference of HAMA1 (anxious mood) scores among patients with different genotypes for 5-HTFLPR polymorphism,where the scores of those with L/S genotype were the highest (2.34±0.80, P= 0.010). (2) The scores of HAMD10 (mental anxiety), HAMA1 (anxious mood), HAMA3 (fear) and mental anxiety factor were higher in patients with 12/10 genotype than patients with 12/12 and 10/10 genotype for STin2 polymorphism (2.40±0.83,2.38±0.90,1.42±1.04,14.60±4.26 respectively; P value :0.014,0.044,0.03 and 0.006 respectively).The scores of HAMD10( mental anxiety) and mental anxiety factor were (2.11±0.77), (12.96±3.78) in the 12/12 genotype patients, and significantly lower than that in the 12/10 genotype patients (adjusted P value: 0.018,0.006).Conclusions A positive association of the 5-HTT polymorphisms with anxious symptoms in depressed patients is revealed. These findings might provide some evidences for the clinical phenotype and optimization of depression treatment.
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2016年第9期797-802,共6页
Chinese Journal of Behavioral Medicine and Brain Science
基金
四川省卫生厅科研项目(100039)