地塞米松对呼吸道合胞病毒感染小鼠肺泡巨噬细胞分化的影响

Influence of dexamethasone on alveolar macrophage differentiation in respiratory syncytial virus infected mice

目的观察地塞米松(dexamethasone,DEX)对小鼠骨髓来源的巨噬细胞(respiratory syncytial virus,BMDM)及呼吸道合胞病毒(RSV)感染小鼠的肺泡巨噬细胞分化的影响。方法体外诱导培养M1型BMDM,鉴定其表型,观察DEX对巨噬细胞转录因子和细胞因子表达的影响;以RSV感染小鼠,同时给予DEX,观察DEX对小鼠肺泡巨噬细胞转录因子和细胞因子表达的影响。结果 DEX能抑制M1型BMDM及RSV感染小鼠的肺泡巨噬细胞中IL-6、IL-12、i NOS和IRF5的表达,并促进IL-10、Arginase-1和IRF4的表达,使M1型巨噬细胞向M2型转化。结论 DEX可在体内外将M1型巨噬细胞转化为M2型巨噬细胞,抑制炎症反应,这可能是DEX治疗RSV感染的作用机制之一。

Objective To observe the influence of dexamethasone on M1 BMDM and alveolar macrophage differentiation in respiratory syncytial virus（ RSV） infected mice. Methods Culture M1 BMDM and identify its phenotype,observe the effect of DEX on its transcription factors and cytokines; In RSV infected mice,observe the influence of DEX on the transcription factors and cytokines of alveolar macrophage. Results DEX can inhibit IL-6,IL-12,i NOS and IRF5 expression,but promote IL-10,Arginase-1 and IRF4 expression in M1 BMDM and alveolar macrophages of RSV infected mice,made M1 macrophages transform to M2 macrophages. Conclusion DEX can transform M1 macrophages to M2 macrophages in vivo and in vitro,and inhibit inflammation,this may be one of the mechanism of DEX in the treatment of RSV infection.