搅拌棒萃取-GC-MS/MS法检测黑蒜汁中8种有机氯农药残留

Determination of Eight Organochlorine Pesticides Residues in Black Garlic Juice by SBSE-GC-MS/MS

建立了搅拌棒萃取-气相色谱法检测黑蒜汁中8种有机氯农药残留。样品经搅拌棒萃取,解析液解析后,上机检测。采用TR-5MS(30 m×0.25 mm×25μm)色谱柱分离,在程序升温的模式下检测,外标法定量。结果表明:8种有机氯在质量浓度0.01~1.0 mg/L范围内线性关系良好,相关系数R^2=0.990 3~0.998 6,方法定量限为0.01 mg/kg。在不同添加水平下,其平均回收率为73.7%~101.6%,变异系数为6.7%~11.5%。该方法简便、快捷,适用于黑蒜汁中有机氯农药残留的检测。

A method was established to determine the 8 organochlorine pesticides residues in black garlic juice by stir bar sorptive extraction（SBSE） combining with gas chromatography tandem mass spectrometer method. The sample was extracted by SBSE. After being desorbed, the sample was performed on TR-5MS（30 m×0.25 mm×25 μm） column. The analysis was carried out in the temperature programming mode and external standard method was used for quantification. The calibration curve of the 8 organochlorine pesticides was good linear from 0.01 to 1.0 mg/L with the correlation coefficient R^2=0.990 3~ 0.998 6, the detection limit of this method was 0.01 mg/kg. In different concentrations, the average recoveries of this method were 73.7%~101.6% and the variability coefficient was 6.7%~11.5%. The method was easy, rapid, and could be applied to the determination of organochlorine pesticides residues in black garlic juice.