摘要
目的:观察电针百会穴对APP/PS1双转基因小鼠葡萄糖代谢水平以及学习记忆的影响,探讨电针治疗阿尔茨海默病的作用机制。方法:将30只雌性12月龄APP/PS1双转基因小鼠随机分为模型组、百会组和非穴组,每组10只,另取10只同窝阴性野生小鼠为野生组。百会组电针百会穴,非穴组电针非穴,每次30min,每天1次,每周5天,共治疗4周,野生组和模型组不干预。采用正电子发射断层扫描技术(PET)观察各组小鼠大脑18F-FDG摄取率的水平;Morris水迷宫实验观察小鼠学习记忆。结果:与模型组相比,百会组小鼠逃避潜伏期缩短(P<0.05),跨越平台次数增加(P<0.01);非穴组小鼠逃避潜伏期与模型组相比无显著性差异(P>0.05);百会组小鼠18F-FDG摄取率高于野生组、模型组以及非穴组。结论:电针百会穴可改善APP/PS1双转基因小鼠的学习记忆功能,其作用机制可能是通过改善小鼠脑内葡萄糖代谢从而发挥神经保护作用。
Objective： To explore effects of electroacupuncture at Baihui （DU20） acupoint on glucose metabolic level and the ability of learning and memory and its underlying mechanism in the brain of APP/PS1 double-transgenic mice. Method： Thirty female APP/PS1 double transgenic mice were randomly divided into model group, DU20 group and non-acupoint group, with 10 mice for each group. And another 10 age-matched wild type mice were experimented as wild group. The DU20 group received electroacupuncture at Baihui （DU20） and the non- acupoint group （APP/PSI＋NA） received electroacupuncture at non-acupoint for 4 weeks （four course of 5 days EA）. Glucose metabolic level of brain was tested by using positron emission tomography scans （PET scans）. Learning and memory ability was tested by Morris water maze （MWM）. Result： Compared with the model group, electroacupuncture at Baihui （DU20） acupoint could ameliorate the learning and memory ability of APP/PS1 double-transgenic mice （P〈0.05）, whereas the times that the mice crossed the location of the platform were increase （P〈0.01）. There was no significant difference between the model group and non-aeupoint group （P〉0.05）. The level of uptake rate of glucose in DU20 group was higher than that in the wild group, model group and non-acupoint group. Conclusion： Electroacupuncture at DU20 acupoint could improve learning and memory ability in APP/PS1 dou- ble-transgenic mice, and the underlying mechanism may be related to the increase of glucose metabolism.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2016年第10期1050-1054,共5页
Chinese Journal of Rehabilitation Medicine
基金
国家自然科学基金项目(81403450)
