低强度全身振动与阿仑膦酸钠防治去势大鼠骨质疏松的对比研究

The different effect between low-magnitude whole-body vibration and alendronate in ovariectomized rats

目的:对比分析低强度全身振动与阿仑膦酸钠防治去势大鼠骨质疏松效果。方法:104只SD大鼠随机分为对照组(sham组)、卵巢摘除术+注射生理盐水组(OVX+VEH组)、卵巢摘除术+全身振动组(OVX+WBV组)和卵巢摘除术+阿仑膦酸钠组(OVX+ALE)。OVX+VEH组是单纯卵巢切除后给予生理盐水(1mg/kg,每周灌胃1次);OVX+WBV组是切除卵巢后给予全身振动(振动强度为0.3g,频率为45—55Hz,每次振动20min,每天1次,每周5次,休息间隔不大于2d,共12周);OVX+ALE组是切除卵巢后给予阿仑膦酸钠(1mg/kg,每周灌胃1次);定期收集标本,测定血清中骨钙素(osteocalcin,OC)和Ⅰ型胶原C端交联肽(CTX)水平,micro-CT检查分析和三点弯曲生物力学检测。结果:血清中OC和CTX在卵巢切除后均增加显著(P<0.001),WBV和ALE治疗组对OC影响不大,但ALE能够显著抑制血清CTX的增加。在实验12周时,与WBV治疗组比,ALE治疗组更能够显著提高胫骨近端松质骨的骨微结构参数[BV/TV(+25.3%,P=0.004),TV apparent(+60.3%,P<0.001),BV material(+28.4%,P=0.044),Tb.N(+15.6%,P=0.001),and Tb.Th(+16.9%,P=0.037)]。在实验12周时,与OVX+WBV治疗组对比时,OVX+ALE治疗组能够提高胫骨的最大载荷、骨的刚度参数和骨的吸收能量,但差异无显著性意义。结论:相对于低强度全身振动,阿仑膦酸钠能够更好的防治去势大鼠骨质疏松和改善骨的微结构。

Objective：To investigate the different effect between low-magnitude whole-body vibration（WBV） and alendro- nate（ALE） on bone properties in ovariectomized rats. Method： A total of 104 SD rats were randomly divided into four groups （sham, OVX＋VEH, OVX＋WBV, OVX＋ALE）. The level of WBV applied was 0.3g at 45-55Hz for 20 rain/d, 5d/week and for 12 weeks. ALE was administered in dose of 1 mg/kg once a week. Every 4 weeks 8 rats from each group were sacrificed and their blood and both tibiae were harvested. The expression of osteocalcin（OC） and C-terminal cross linked telopeptides of type I collagen（CTX） in serum was measured by enzyme-linked immunosorbent assay （ELI- SA） and the tibiaes were subjected to metaphyseal three-point bending and micro-CT analysis. Result： OC rose after ovariectomy and was not appreciably changed by either ALE or WBV alone or in com- bination. ALE treatment significantly prevented an increase of CTX. Compared with the OVX＋WBV group,nearly all tested indices such as the BV/TV, TV apparent, Tb.N, Tb.Th, and Conn.D were higher in the OVX＋ ALE group at week 12. At week 12, tibiae treated with ALE exhibited a significantly higher Fma~, energy ab- sorption and stiffness compared to the OVX＋WBV group, but there was no significant difference. Conclusion： Compared with the WBV, ALE was more effective at preventing bone loss and improved becular architecture.