简便三波长分光光度法测定药物及生物样品中呋塞米

Determination of furosemide in drug and biological samples by simple and convenient triple-wavelength spectrophotometry

在酸性Tris-盐酸缓冲介质中,孔雀石绿与呋塞米反应生成了具有3个峰值相近的负吸收峰的绿色离子缔合物,其波长分别位于652,554和424 nm,表观摩尔吸光系数分别为2.21×10^4,2.28×10^4和1.96×10^4L/（mol·cm）。当用三波长法测定时,表观摩尔吸光系数（ε）可达6.45×10^4L/（mol·cm）。呋塞米的质量浓度在0^5.1 mg/L内遵从比尔定律。方法适用于人体血液、尿液及药物中呋塞米的测定。

In an acidic tris-hydrochloric acid buffer medium,malachite green reacts with furosemide to form a green ion association complex with obvious three negative absorption peaks with very close height located at 652,554 and 424 nm respectivelywith very close and the wavelengths. Their apparent molar absorptivities（ ε） were2. 21 ×10^42. 28 ×10^4 and 1. 96 ×10^4 L/（ mol · cm）,respectively. When the 3-wavelength superposition absorption spectrometry was used to determine the concentration of furosemide,its apparent molar absorptivity（ ε） was estimated to be 6. 45 ×10^4 L/（ mol·cm）. Beer＇s law was obeyed in definite mass concentration range within 0 ^ 5. 1 mg/L of furosemide. The method was applied to determine the content of furosemide in commercially available furosemide medicine,human blood and human urine with satisfactory results.