冷应激前后大鼠肺脏、肾脏、肌肉及十二指肠α-烯醇化酶基因的定量

Quantitative determination of expression level of α-enolase in lung,kidney, muscle and duodenum of rats before and after cold stress

目的研究急性冷刺激对大鼠肺脏、肾脏、肌肉及十二指肠α-烯醇化酶(α-enolase,ENO1)基因表达量的影响。方法取健康大鼠10只,随机分为2组:冷应激组和正常对照组,每组5只。正常对照组饲养环境为24℃,冷应激组大鼠环境温度为4℃,应激时间为12 h。实验后分别取两组大鼠肺脏、肾脏、肌肉及十二指肠组织样品,应用实时荧光定量PCR检测应激前后大鼠四种内脏器官ENO1 m RNA表达量。结果冷应激组肺脏与十二指肠ENO1 m RNA表达水平显著高于正常对照组(P<0.05);肾脏与肌肉ENO1 m RNA表达水平显著下降(P<0.05)。结论急性冷刺激可通过影响肺脏、肌肉、肾脏及十二指肠ENO1基因表达调节代谢燃料的选择和应激反应程度。

Objective To investigate the effect of cold stress on expression level of α-enolase（ENO1） in lung, kidney,muscle and duodenum of rats. Methods Ten healthy male Wistar rats were randomly divided into cold stress and normal control groups, five for each. The rats in normal control group were raised at 24 ℃, while those in cold stress group at 4 ℃for 24 h. The lung, kidney, muscle and duodenum of rats in two groups were collected and determined for expression level of ENO1 m RNA by real-time fluorescent quantitative PCR. Results The expression levels of ENO1 m RNA in lung and duodenum of rats in cold stress group were significantly higher than those in control group（P〈0. 05）, while those in kidney and muscle decreased significantly（P〈0. 05）. Conclusion Acute cold stress regulated the metabolic fuel selection and stress response by affecting the expression of ENO1 gene in lung, muscle, kidney and duodenum.