超滤膜牛血清蛋白截留率测定的不确定度评定

Evaluation of Uncertainty in Determination of the UF Membrane Rejection Rate of Bovine Serum Albumin

分析了采用分光光度法测定超滤膜牛血清蛋白截留率过程中不确定度的来源,包括标准物质、牛血清蛋白测试溶液制备、校准曲线回归、测试液和超滤产水稀释过程、重复性测量、相关性等因素引入的不确定度。演示了其计算方法,最后合成标准不确定度,乘以扩展因子2,获得测量结果的扩展不确定度。不确定度的主要来源为牛血清蛋白标准物质的不确定度、校准曲线引入的不确定度和重复性测量的不确定度。

The sources of uncertainty were analyzed that determination of the UF membrane rejection rate of BSA by UV spectrophotometry. The calculation of uncertainties were demonstrated,including standard material,preparation of BSA test solution,calibration curve,dilution process of the test solution and the product water,repetition of measurement and correlation factor. The synthetic standard uncertainty and extended uncertainty was calculated. The main sources of uncertainty are the standard material,the calibration curve and the repetition of measurement.