补阳还五汤提取过程中最佳提取时间和药材粉碎度的研究

The Research of Optimum Extraction Time and Degree of Crushing Herbs of Buyang Huanwu Decoction

目的研究补阳还五汤提取过程中的最佳提取时间和药材粉碎度。方法显微观察不同粉碎度的补阳还五汤单味药材,观察药材的破壁情况,采用HPLC分析补阳还五汤不同粉碎度药材的成分溶出情况。结果显微观察从80目到100目,药材细胞结构已经不完整,细胞壁开始破壁。提取过程的中段,不同粉碎度之间的有效成分共有峰的个数较多,溶出比较完全。在提取时间约300 min时,不同目数指纹图谱的相似度在0.85以上。结论 20目药材在240 min时、40目药材在180 min时、60目在120 min时、80目药材在90 min时、100目药材在30 min时、饮片在180 min时补阳还五汤的溶出达到基本稳定,提取效果最佳。

Objective To determine the optimum extraction time and particle size of Buyang Huaiwu Decoction（BYHWD）. Methods The damage of different grinding degrees BYHWD cell wall was determined by microscopy and the active ingredients dissolution situation herbs broken were analyzd by HPLC. Results The microstructure of herbs at 80 mesh to 100 mesh was messy and irregular. Herbs cell structure was incomplete because of broking of cell wall. In the middle of the extraction process, the common peaks of active ingredients from different grinding degrees herbs are the most. The similarity of different fingerprints was basically above 0.85 at the extraction time of 300 min. Conclusion The dissolution of BYHWD is stable at 20 mesh/240 min, 40 mesh/180 min, 60 mesh/120 min, 80 mesh/90 min, 100 mesh/30 min and decoction pieces and the extraction effect is best.