PP纤维支撑蛋白质印迹海藻酸钙凝胶膜的制备和表征

Preparation and characterization of PP non-woven fiber supported protein molecular imprinted calcium alginate hydrogel film

以海藻酸钠(Na Alg)为印迹材料,以牛血清白蛋白(BSA)为模板分子,以聚丙烯(PP)无纺布为支撑体,制备了PP无纺布支撑的BSA分子印迹海藻酸钙水凝胶膜(PP-s-Ca Alg MIP).同时不加蛋白质制备了非印迹膜,记为PP-s-Ca Alg NIP.通过扫描电镜(SEM)、红外光谱(FT-IR)对PP-s-Ca Alg凝胶膜进行表征,测定了其在生理盐水中的溶胀性能.讨论了不同Na Alg浓度制备的PP-s-Ca Alg水凝胶膜在不同缓冲液中对BSA的释放.研究了不同缓冲液下不同Na Alg浓度制备的PP-s-Ca Alg MIP膜和NIP膜对BSA的吸附性能.结果表明:PP-s-Ca Alg MIP膜对BSA的吸附量明显优于PP-s-Ca Alg NIP膜;与对比蛋白质相比,PP-s-Ca Alg MIP对模板蛋白质的吸附量更多,表明其具有一定的吸附选择性;PP-s-Ca Alg MIP膜制备简单,成本低,生物相容性好,可用于细胞培养和组织工程领域.

Bovine serum albumin （BSA） imprinted calcium alginate hydrogel film supported by polypropylene （PP） non- woven fabrics （PP-s-CaAlg MIP） was prepared using sodium alginate as the molecularly imprinted materials,BSA as the template molecule, and PP non-woven fabrics as the matrix. At the same time the non-molecularly imprinted film was also prepared without BSA and named as PP-s-CaAlg NIP. The PP-s-CaAlg MIP was char-acterized by SEM and FT-IR, and its swelling performance was measured in physiological saline. The release properties of BSA on the PP-s-CaAlg hydrogel films prepared with different NaAlg concentrations were investi-gated in different buffers. The adsorption properties of the PP-s-CaAlg MIP and NIP films prepared with different NaAlg concentrations under different buffers were analyzed. The experimental results showed that the PP -s - CaAlg MIP film adsorbed more BSA than PP-s-CaAlg NIP film. Compared with contrast protein, PP-s-CaAlg MIP adsorbed more template protein, indicating that the PP-s-CaAlg MIP exhibited the performance of adsorp-tion selectivity. PP-s-CaAlg MIP exhibited good biocompatibility and could be prepared by a fast, low -cost method, which would find its potential applications in the fields of cell culture and tissue engineering.